| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 2.7.11.1; Nuclear mitogen- and stress-activated protein kinase 1; 90 kDa ribosomal protein S6 kinase 5; RSK-like protein kinase; RSKL |
| Entrez GeneID | 9252; |
| WB Predicted band size | 90kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from Internal of human MSK1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MSK1 (Ab-212)抗体的3篇参考文献及其摘要概览：
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1. **文献名称**: *Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38. and may mediate activation of CREB*
**作者**: Deak, M., et al. (1998)
**摘要**: 该研究首次鉴定了MSK1作为MAPK和SAPK2/p38的下游激酶，并发现其通过磷酸化转录因子CREB参与基因表达调控。文献中使用了MSK1 (Ab-212)抗体进行Western blot分析，验证了MSK1在细胞应激和生长因子刺激下的激活机制。
2. **文献名称**: *MSK1 activity is controlled by multiple phosphorylation sites*
**作者**: McCoy, C.E., et al. (2005)
**摘要**: 本文通过质谱分析和点突变技术，系统研究了MSK1的多个磷酸化位点对其激酶活性的调控作用。研究利用MSK1 (Ab-212)抗体进行免疫沉淀实验，证实了其在信号转导中的动态磷酸化修饰与功能关联。
3. **文献名称**: *Chromatin modifications by MSK1 link inflammatory signaling to gene expression*
**作者**: Soloaga, A., et al. (2003)
**摘要**: 该文献揭示了MSK1通过磷酸化组蛋白H3(Ser10)介导染色质重塑，从而连接炎症信号与基因转录。研究采用MSK1 (Ab-212)抗体进行免疫荧光染色，证明了MSK1在细胞核内的定位及其与炎症因子的共激活作用。
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**说明**：上述文献为基于MSK1功能研究的代表性论文，其中Ab-212抗体多用于Western blot、免疫沉淀或细胞定位实验。若需具体产品验证数据，建议参考抗体供应商(如Cell Signaling Technology)的技术文档或应用指南。
MSK1 (Ab-212) is a monoclonal antibody specifically designed to recognize mitogen- and stress-activated protein kinase 1 (MSK1), a serine/threonine kinase involved in critical cellular processes. MSK1. encoded by the *RPS6KA5* gene, acts downstream of the ERK and p38 MAPK signaling pathways, playing a role in regulating transcription through phosphorylation of targets like CREB and histone H3. It is implicated in inflammation, cell survival, and stress responses, making it a focus in cancer and neurological disease research.
The Ab-212 clone targets endogenous MSK1 proteins across various applications, including Western blotting, immunoprecipitation, and immunofluorescence. Its specificity for human MSK1. confirmed through knockout/knockdown validation, ensures reliable detection of both phosphorylated and unmodified forms, aiding studies on kinase activation dynamics. Developed in murine hosts, this antibody is widely used to investigate MSK1’s role in MAPK signaling crosstalk, chromatin remodeling, and disease mechanisms. Researchers leverage it to explore therapeutic targeting of MSK1 in pathologies linked to dysregulated kinase activity, such as tumors or neurodegenerative disorders. Its robust performance in diverse experimental setups underscores its utility in elucidating MSK1-mediated signaling networks.
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