| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | C-COT; cancer Osaka thyroid oncogene; COT proto-oncogene serine/threonine-protein kinase; EC 2.7.11.25; kinase Cot |
| Entrez GeneID | 1326; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized non-phosphopeptide derived from human MAP3K8 around the phosphorylation site of serine 400 (C-Q-S(p)-L-D). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MAP3K8 (Ab-400)抗体的虚构参考文献示例(仅供参考，实际文献需通过学术数据库查询)：
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1. **文献名称**：*MAP3K8 regulates inflammatory signaling in macrophages through NF-κB activation*
**作者**：Smith J, et al.
**摘要**：本研究利用MAP3K8 (Ab-400)抗体，通过Western blot和免疫组化分析了MAP3K8在巨噬细胞中的表达及其对NF-κB信号通路的调控作用，揭示了其在炎症反应中的关键功能。
2. **文献名称**：*Oncogenic role of MAP3K8 in melanoma progression*
**作者**：Lee H, et al.
**摘要**：通过免疫沉淀(使用Ab-400抗体)和基因沉默技术，作者发现MAP3K8通过激活ERK通路促进黑色素瘤细胞侵袭，提示其作为潜在治疗靶点。
3. **文献名称**：*MAP3K8 deficiency alters cardiac hypertrophy pathways*
**作者**：Garcia R, et al.
**摘要**：该研究在小鼠模型中结合Ab-400抗体检测MAP3K8蛋白水平，发现其缺失通过调节JNK信号抑制病理性心脏肥大，为心血管疾病机制提供了新见解。
4. **文献名称**：*Validation of MAP3K8 antibody specificity in kinase activity assays*
**作者**：Chen L, et al.
**摘要**：文章系统验证了Ab-400抗体的特异性，证明其适用于检测内源性MAP3K8的激酶活性及亚细胞定位，为相关研究提供了技术参考。
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**注意**：以上内容为模拟生成，实际文献需通过**PubMed/Google Scholar**搜索关键词“MAP3K8 antibody Ab-400”或联系抗体供应商(如Santa Cruz Biotechnology等)获取产品引用文献。若需具体文献，建议补充抗体生产商或货号信息。
The MAP3K8 (Ab-400) antibody is designed to detect the mitogen-activated protein kinase kinase kinase 8 (MAP3K8), also known as Tpl2 (tumor progression locus 2) or Cot (cancer Osaka thyroid). MAP3K8 is a serine/threonine kinase involved in critical signaling pathways, including the NF-κB and MAPK/ERK cascades, which regulate immune responses, inflammation, and cell proliferation. The Ab-400 epitope typically corresponds to a phosphorylated residue (Ser400) in the kinase domain, reflecting its activation state. This antibody is commonly used in research to study MAP3K8's role in diseases like cancer, autoimmune disorders, and inflammatory conditions, where aberrant kinase activity is linked to pathogenesis.
Produced in rabbits as a polyclonal or monoclonal antibody (depending on the supplier), it is validated for applications such as Western blotting, immunoprecipitation, and immunohistochemistry. It often demonstrates reactivity with human, mouse, and rat MAP3K8 homologs. Researchers use this tool to investigate phosphorylation-dependent signaling mechanisms or to assess MAP3K8 expression levels in experimental models. Specificity is confirmed through knockout controls or peptide-blocking assays. Its utility extends to preclinical studies targeting MAP3K8 inhibition, as the kinase is a potential therapeutic target in oncology and chronic inflammation. Proper storage and optimized dilution protocols are critical for maintaining antibody performance.
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