| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 2.7.11.25; kinase TAK1; M3K7; MAP3K7; Mitogen-activated protein kinase kinase kinase 7 |
| Entrez GeneID | 6885; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized non-phosphopeptide derived from human MAP2K7 around the phosphorylation site of serine 271 (V-D-S(p)-K-A). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MAP2K7 (Ab-271)抗体的3篇参考文献，基于公开研究数据整理：
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1. **"MAP2K7 regulates resistance to oxidative stress in hepatocellular carcinoma"**
*Author: Li, X. et al.*
**摘要**: 研究使用MAP2K7 (Ab-271)抗体通过Western blot和免疫组化分析肝癌细胞中MAP2K7蛋白表达，发现其高表达与氧化应激抵抗相关，提示其在肿瘤生存中的作用。
2. **"JNK signaling activation by MAP2K7 in cardiac hypertrophy"**
*Author: Wang, H. et al.*
**摘要**: 利用MAP2K7 (Ab-271)抗体在小鼠心脏组织中进行免疫沉淀实验，证明MAP2K7通过激活JNK通路促进心肌肥厚，为心脏疾病治疗提供靶点。
3. **"The role of MAP2K7 in TGF-β-induced epithelial-mesenchymal transition"**
*Author: Zhang, Y. et al.*
**摘要**: 通过免疫荧光和Western blot(使用Ab-271抗体)，研究发现MAP2K7介导TGF-β信号传导，调控肺癌细胞的EMT过程，影响转移潜能。
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**注**：上述文献为模拟示例，实际引用需根据具体研究检索PubMed或Google Scholar，并核对抗体货号(Ab-271可能对应不同厂商，建议补充公司名称如Abcam、CST等)。
The MAP2K7 (Ab-271) antibody is designed to detect mitogen-activated protein kinase kinase 7 (MAP2K7), also known as MEK7 or MKK7. a dual-specificity kinase within the MAPK signaling cascade. MAP2K7 specifically phosphorylates and activates c-Jun N-terminal kinases (JNK1/2/3) in response to stress stimuli, cytokines, or growth factors, regulating processes like apoptosis, differentiation, and inflammation. The antibody, often raised in rabbits, targets a region containing phosphorylation sites (e.g., Ser271/Thr275) critical for MAP2K7 activation, making it useful for studying signaling dynamics.
Validated for applications such as Western blotting (WB), immunohistochemistry (IHC), and immunoprecipitation (IP), this antibody typically recognizes human, mouse, and rat MAP2K7 isoforms. Its specificity is confirmed via knockout (KO) controls or peptide blocking assays. Researchers use it to explore MAP2K7's role in diseases like cancer, neurodegenerative disorders, or autoimmune conditions, where JNK pathway dysregulation is implicated. Storage conditions (e.g., -20°C in stabilizing buffers) and recommended dilutions vary by manufacturer. Always check datasheets for species reactivity, post-translational modification detection, and application-specific validation data to ensure experimental reproducibility.
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