Rabbit Polyclonal Smad2(Ab-467) Antibody

The Smad2 (Ab-467) antibody is a widely used reagent in studies investigating the transforming growth factor-beta (TGF-β) signaling pathway. Smad2. a member of the receptor-regulated Smad (R-Smad) family, plays a critical role in mediating TGF-β signal transduction. Upon TGF-β receptor activation, Smad2 is phosphorylated at specific C-terminal serine residues (e.g., Ser465/467 in humans), leading to its association with Smad4 and subsequent nuclear translocation to regulate gene expression. The Smad2 (Ab-467) antibody specifically detects Smad2 phosphorylated at Ser467 (or equivalent residues in other species), making it a valuable tool for studying TGF-β pathway activation. It is commonly employed in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to assess Smad2 activation status in various cellular and tissue contexts. This antibody is particularly useful in research areas like cancer, fibrosis, and developmental biology, where dysregulated TGF-β signaling is implicated. Its specificity for the phosphorylated (active) form of Smad2 allows researchers to distinguish between inactive and TGF-β-activated states, providing insights into pathway dynamics. Validation typically includes testing in knockout models or phosphorylation-inhibited conditions to confirm target specificity.