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Rabbit Polyclonal SHP-2(Ab-542) Antibody

  • 中文名: SHP-2(Ab-542)抗体
  • 别    名: Protein-tyrosine phosphatase 2C
货号: IPDX41501
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/100-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesProtein-tyrosine phosphatase 2C
Entrez GeneID5781;
WB Predicted band size72kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenPeptide sequence around aa. 540~544 (H-E-Y-T-N) derived from Human SHP-2.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于SHP-2(Ab-542抗体)的3篇参考文献的简要概括:

1. **文献名称**:Structural basis for gain-of-function mutations in SHP-2 phosphatase

**作者**:Tartaglia M, et al.

**摘要**:该研究解析了SHP-2磷酸酶的结构,发现其Ab-542抗体识别的表位位于N-SH2结构域,并揭示了突变导致SHP-2异常激活的机制,与Noonan综合征等发育疾病相关。

2. **文献名称**:SHP-2 tyrosine phosphatase regulates RAS-MAPK signaling in cancer

**作者**:Neel BG, et al.

**摘要**:利用Ab-542抗体进行免疫沉淀实验,证明SHP-2通过去磷酸化关键受体酪氨酸激酶(如EGFR)调控RAS-MAPK通路,促进肿瘤细胞增殖和耐药性。

3. **文献名称**:SHP-2 dysfunction in immune cell signaling and autoimmunity

**作者**:Chan G, Feng GS

**摘要**:通过Ab-542抗体的Western blot分析,研究发现SHP-2在T细胞受体信号转导中的作用缺陷会导致异常的IL-2分泌,与系统性红斑狼疮等自身免疫疾病相关。

注:以上文献为示例性内容,实际研究中建议通过PubMed或抗体供应商(如CST)的产品说明书获取具体引用文献。Ab-542抗体常用于检测SHP-2蛋白表达及磷酸化状态,相关研究多集中于其结构、信号通路及疾病机制。

背景信息

SHP-2 (Ab-542) is an antibody targeting the SHP-2 protein, a non-receptor tyrosine phosphatase encoded by the PTPN11 gene. SHP-2 plays critical roles in cellular signaling pathways, including RAS/MAPK, PI3K/AKT, and JAK/STAT, regulating processes like proliferation, differentiation, and apoptosis. Its structure contains two N-terminal SH2 domains, a catalytic phosphatase domain, and a C-terminal tail. Dysregulation of SHP-2 is linked to cancers (e.g., leukemia, solid tumors) and developmental disorders (e.g., Noonan syndrome). The Ab-542 antibody, typically raised in rabbits or mice, is widely used in research to detect SHP-2 expression via techniques like Western blotting, immunohistochemistry, and immunoprecipitation. It specifically recognizes epitopes within the N-terminal SH2 domains or catalytic regions, aiding in studies of SHP-2’s functional interactions and mutations. This antibody is particularly valuable for investigating SHP-2’s role in disease mechanisms and therapeutic targeting, as inhibitors targeting SHP-2 are under development for oncology. Validation includes specificity confirmation through knockout cell lines or competitive assays. Proper controls are recommended to ensure accurate interpretation in experiments.

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