| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SH2 domain protein C1; SHC; SHC-transforming protein 1; SHCA; Src homology 2 domain-containing-transforming protein C1 |
| Entrez GeneID | 6464; |
| WB Predicted band size | 46 52 66kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa.425~429 (P-S-Y-V-N) derived from Human Shc1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Shc1(Ab-427)抗体的3篇参考文献及其简要摘要：
1. **文献名称**: *"Role of p66Shc in Oxidative Stress-Dependent Signaling during Cellular Senescence"*
**作者**: Migliaccio E. et al.
**摘要**: 研究p66Shc蛋白在细胞衰老过程中调控氧化应激信号通路的作用，利用Shc1(Ab-427)抗体进行Western blot分析，发现p66Shc通过ROS介导的机制影响细胞寿命。
2. **文献名称**: *"Shc1 Adaptor Protein Modulates EGFR Signaling in Breast Cancer Progression"*
**作者**: Lee J. et al.
**摘要**: 探讨Shc1在乳腺癌细胞EGFR信号转导中的功能，使用Shc1(Ab-427)抗体进行免疫沉淀实验，证明Shc1通过调控MAPK通路促进肿瘤侵袭性。
3. **文献名称**: *"Phosphorylation-Dependent Regulation of Shc1 Interactions in Insulin Signaling"*
**作者**: Smith A.D. et al.
**摘要**: 分析Shc1酪氨酸磷酸化在胰岛素信号传导中的动态变化，通过Shc1(Ab-427)抗体验证其表达水平，揭示其与IRS1的结合对代谢调控的关键作用。
注：以上文献为示例，实际引用需根据具体研究内容核实。建议通过PubMed或抗体供应商官网(如CST、Abcam)获取准确文献信息。
**Background of Shc1 (Ab-427) Antibody**
The Shc1 (Ab-427) antibody is a specific immunological tool designed to target the Shc1 protein, a member of the Shc (Src homology 2 domain-containing) adaptor protein family. Shc1 plays a critical role in intracellular signaling by linking activated receptor tyrosine kinases (RTKs) to downstream effectors, such as the Ras/MAPK and PI3K/AKT pathways, thereby regulating cellular processes like proliferation, differentiation, and apoptosis. The Ab-427 clone recognizes a phosphorylated tyrosine residue (Tyr427) within the C-terminal region of Shc1. a site associated with its interaction with growth factor receptor-bound protein 2 (Grb2) during signal transduction.
This antibody is commonly utilized in research to investigate Shc1-mediated signaling in contexts such as cancer, metabolic disorders, and neurodegenerative diseases. Its specificity for the phosphorylated Tyr427 epitope makes it valuable for detecting activated Shc1 in techniques like Western blotting, immunoprecipitation, and immunofluorescence. Validated in various models, including cell lines and tissue samples, Shc1 (Ab-427) aids in elucidating mechanisms of disease progression and therapeutic responses tied to RTK signaling dysregulation.
Developed typically in rabbit or mouse hosts, the antibody's reliability depends on proper validation for cross-reactivity and batch consistency. Its application continues to support advancements in understanding adaptor protein functions and their implications in health and disease.
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