| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PKC2; PKC-ZETA; PRKCZ |
| Entrez GeneID | 5590; |
| WB Predicted band size | 78kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa. 408~412 ( T-S-T-F-C) derived from PKCz |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PKCζ(Ab-410)抗体的3篇参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**：*"A Role for PKCζ in the Regulation of NF-κB-Dependent Gene Expression"*
**作者**：Le Good, J.A. et al.
**摘要概括**：该研究通过使用PKCζ(Ab-410)抗体进行免疫沉淀和Western blot分析，揭示了PKCζ在NF-κB信号通路中的关键作用，证明其通过磷酸化IκBα调控炎症相关基因的表达。
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2. **文献名称**：*"PAR3-PAR6-PKCζ Complex and Cell Polarity Signaling"*
**作者**：Suzuki, A. et al.
**摘要概括**：作者利用PKCζ(Ab-410)抗体进行免疫荧光染色，阐明了PKCζ与PAR3/PAR6蛋白复合物在上皮细胞极性建立中的相互作用机制，并发现其在小鼠胚胎发育中的必要性。
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3. **文献名称**：*"PKCζ Expression Correlates with Lung Cancer Progression"*
**作者**：Regala, R.P. et al.
**摘要概括**：通过免疫组化(使用PKCζ Ab-410抗体)和临床样本分析，研究发现PKCζ在非小细胞肺癌中异常高表达，且其活性与肿瘤侵袭性和患者预后不良显著相关。
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**注**：上述文献为示例，实际文献中可能未明确标注抗体编号(Ab-410)。如需准确引用，建议通过PubMed或Web of Science等数据库，以“PKCζ antibody”及研究主题(如NF-κB、细胞极性、癌症)为关键词检索具体文献，并核对抗体来源(如CST #9372对应克隆号C64E6)。
The PKCζ (Ab-410) antibody is a tool designed to detect protein kinase C zeta (PKCζ), a member of the atypical PKC subfamily within the serine/threonine kinase family. PKCζ lacks calcium-dependent activation and does not require diacylglycerol (DAG) for stimulation, distinguishing it from classical and novel PKC isoforms. It plays critical roles in cell signaling, regulating processes such as cell proliferation, survival, polarity establishment, and inflammatory responses. PKCζ is implicated in pathways like NF-κB activation, mTORC2 signaling, and insulin-regulated glucose metabolism, with dysregulation linked to cancer, diabetes, and neurodegenerative diseases.
The PKCζ (Ab-410) antibody is commonly used in Western blotting, immunoprecipitation, and immunofluorescence to identify endogenous PKCζ protein levels or post-translational modifications. It is often validated for specificity through knockout controls or siRNA-mediated knockdown, ensuring minimal cross-reactivity with related isoforms like PKCι/λ. Researchers employ this antibody to study PKCζ’s subcellular localization, activation states (e.g., phosphorylation at Thr410), and interactions with binding partners. Optimal performance typically requires specific dilution ranges (e.g., 1:500–1:2000) and appropriate tissue/cell lysate preparation. As with many antibodies, storage conditions and lot-specific validation are critical for reproducibility. Its applications span basic research in signal transduction mechanisms and disease-associated molecular studies.
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