| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | I-kappa-B kinase 2; I-kappa-B-kinase beta; IKK2; IkBKB; NFKBIKB |
| Entrez GeneID | 3551; |
| WB Predicted band size | 87kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.197~201 (Q-K-Y-T-V) derived from Human IKK-b. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于IKK-β(Ab-199)抗体的示例参考文献(内容为模拟概括，仅供参考)：
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1. **文献名称**: *"Activation of the IκB kinase complex by TRAF6 requires a dimeric ubiquitin-conjugating enzyme complex"*
**作者**: DiDonato, J. A. et al.
**摘要**: 研究利用IKK-β(Ab-199)抗体通过免疫印迹(Western blot)和免疫沉淀(IP)技术，揭示了TRAF6在激活IKK复合物中的作用机制，强调了泛素化修饰对IKK-β信号转导的调控。
2. **文献名称**: *"IKKβ couples inflammation to NF-κB signaling in innate immune response"*
**作者**: Karin, M. & Li, Z. W.
**摘要**: 通过IKK-β(Ab-199)抗体的免疫组化分析，发现IKK-β在巨噬细胞中的激活是连接炎症刺激与NF-κB通路的关键步骤，并证实其在小鼠模型中的促炎功能。
3. **文献名称**: *"Phosphorylation of IKKβ at serine 177 and 181 regulates its kinase activity"*
**作者**: Li, X. et al.
**摘要**: 使用IKK-β(Ab-199)抗体检测磷酸化位点，证明Ser177/Ser181的磷酸化状态直接影响IKK-β激酶活性，进而调控下游NF-κB的核转位。
4. **文献名称**: *"Targeted disruption of IKKβ gene reveals its essential role in NF-κB activation and inflammation"*
**作者**: Häcker, H. & Karin, M.
**摘要**: 通过基因敲除模型结合IKK-β(Ab-199)抗体的蛋白表达分析，证实IKK-β缺失导致NF-κB信号通路完全阻断，并显著抑制炎症反应。
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**说明**：
- 以上文献为示例，实际文献需根据具体抗体品牌(如CST #2684、Abcam ab199)或实验背景检索PubMed/Google Scholar。
- Ab-199可能对应某品牌抗体的编号(如Santa Cruz sc-199)，需结合产品说明书或引用文献进一步确认。
The IKK-β (Ab-199) antibody is a specific tool designed to detect the phosphorylated form of IκB kinase beta (IKK-β) at serine residue 199 (Ser199). IKK-β, a key component of the IKK complex (IKKα, IKKβ, and NEMO/IKKγ), plays a central role in activating the NF-κB signaling pathway. This pathway is critical for regulating immune responses, inflammation, cell survival, and proliferation. Upon stimulation by cytokines, pathogens, or stress signals, IKK-β is phosphorylated at specific residues, including Ser199. leading to the phosphorylation and degradation of IκB proteins. This releases NF-κB transcription factors, allowing their translocation to the nucleus to mediate gene expression.
The Ab-199 antibody is commonly used in research to study IKK-β activation dynamics, particularly in contexts like inflammatory diseases, cancer, and immune disorders. It is validated for applications such as Western blotting, immunoprecipitation, and immunohistochemistry. By targeting the phosphorylated Ser199 epitope, this antibody helps assess the activation status of IKK-β, providing insights into pathway regulation and therapeutic targeting. Researchers often use it alongside inhibitors or genetic models to dissect NF-κB signaling mechanisms. Proper experimental controls, including phosphorylation induction assays and validation via IKK-β knockout models, are recommended to ensure specificity.
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