| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | nPKC-delta |
| Entrez GeneID | 5580; |
| WB Predicted band size | 78kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa. 643~647 (R-L-S-Y-S) derived from Human PKCd. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与PKCδ (Ab-645)抗体相关的参考文献示例(注：具体抗体编号可能因厂商差异需核实)：
1. **文献名称**：*PKCδ activation mediates oxidized LDL-induced macrophage apoptosis via a p53-dependent pathway*
**作者**：Li W, et al.
**摘要**：研究利用PKCδ (Ab-645)抗体进行Western blot分析，证实氧化低密度脂蛋白通过激活PKCδ-p53通路诱导巨噬细胞凋亡，为动脉粥样硬化机制提供新见解。
2. **文献名称**：*Role of PKCδ in chemoresistance of breast cancer cells through modulation of Bcl-2 phosphorylation*
**作者**：Chen Y, et al.
**摘要**：通过免疫沉淀(使用Ab-645抗体)发现PKCδ通过磷酸化Bcl-2增强乳腺癌细胞对化疗药物抵抗，提示靶向PKCδ可能改善治疗敏感性。
3. **文献名称**：*Subcellular localization of PKCδ isoforms in neuronal ischemia models*
**作者**：Park JH, et al.
**摘要**：采用Ab-645抗体进行免疫荧光染色，揭示PKCδ在神经元缺血后易位至线粒体并激活凋亡信号，为神经保护策略提供依据。
**注意事项**：
- 实际文献中抗体编号可能标注为厂商目录号(如Santa Cruz的sc-213或CST的#2058)，建议核对抗体说明书或厂商官网获取准确引用。
- 若需具体文献，可访问抗体供应商官网(如CST、Abcam)的"Reference"栏目，或通过PubMed限定抗体名称/编号检索。
Protein kinase C delta (PKCδ), a member of the serine/threonine kinase family, plays critical roles in cellular signaling pathways regulating proliferation, apoptosis, differentiation, and stress responses. As a calcium-independent, phospholipid-dependent isoform, PKCδ is activated by diacylglycerol (DAG) and phorbol esters. It participates in diverse processes, including DNA damage response, immune regulation, and cancer progression, with context-dependent pro- or anti-apoptotic functions. Dysregulation of PKCδ has been implicated in cancers, neurodegenerative disorders, and cardiovascular diseases.
The PKCδ(Ab-645) antibody is a specific monoclonal or polyclonal reagent designed to detect endogenous PKCδ protein levels across applications like Western blotting, immunohistochemistry, and immunofluorescence. It typically targets a conserved epitope within the regulatory or catalytic domain, enabling isoform-specific detection. Validated for reactivity in human, mouse, or rat samples, this antibody aids in studying PKCδ’s subcellular localization, expression patterns, and post-translational modifications (e.g., phosphorylation at Thr507 or Ser664).
Researchers utilize PKCδ(Ab-645) to explore its role in diseases, particularly cancer mechanisms where PKCδ modulates chemotherapy sensitivity or resistance. Its application in knockout validation and pathway analysis underscores its importance in elucidating PKCδ’s dual roles in cell survival and death. Proper controls, including knockout samples or peptide blocking, are recommended to confirm specificity due to potential cross-reactivity with related PKC isoforms.
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