| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MACS; MARCS; PKCSL; PRKCSL; Protein kinase C substrate |
| Entrez GeneID | 4082; |
| WB Predicted band size | 80kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.156~160 (R-F-S-F-K) derived from Human MARCKS. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MARCKS (Ab-158)抗体的参考文献示例(内容为虚构，仅作格式参考)：
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1. **文献名称**: *MARCKS蛋白在乳腺癌细胞迁移中的功能研究*
**作者**: Smith J, et al.
**摘要**: 本研究利用MARCKS (Ab-158)抗体进行Western blot和免疫荧光分析，发现MARCKS在乳腺癌细胞中高表达，并通过调节细胞膜动态参与肿瘤迁移。实验表明，抑制MARCKS磷酸化可降低癌细胞侵袭能力。
2. **文献名称**: *MARCKS调控巨噬细胞炎症反应的分子机制*
**作者**: Johnson L, et al.
**摘要**: 通过MARCKS (Ab-158)抗体的免疫共沉淀技术，研究者发现MARCKS与TLR4信号通路相互作用，调控巨噬细胞中促炎因子释放。结果表明，MARCKS缺失会显著抑制炎症反应。
3. **文献名称**: *MARCKS在神经突触可塑性中的表达变化*
**作者**: Lee S, et al.
**摘要**: 使用MARCKS (Ab-158)抗体进行小鼠脑组织免疫组化分析，发现其在海马区突触后膜富集。研究提示MARCKS可能通过钙调蛋白依赖性途径参与学习记忆过程。
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**注**：以上为模拟示例，实际文献需通过学术数据库(如PubMed、Google Scholar)以关键词“MARCKS antibody Ab-158”或相关研究领域进行检索。建议结合实验需求筛选涉及该抗体具体应用(如WB、IF、IP等)的文献。
The MARCKS (Myristoylated Alanine-Rich C-Kinase Substrate) protein is a ubiquitously expressed intracellular substrate of protein kinase C (PKC) and plays critical roles in cell motility, membrane trafficking, and actin cytoskeleton dynamics. It acts as a crosslinker between the plasma membrane and cytoskeletal networks, regulating processes like neurosecretion, phagocytosis, and inflammatory responses. The MARCKS(Ab-158) antibody is a specific monoclonal antibody raised against epitopes within the human MARCKS protein. It is widely used in research to detect MARCKS expression and phosphorylation status via techniques such as Western blotting, immunohistochemistry, and immunofluorescence.
This antibody has been particularly valuable in studying MARCKS-related signaling pathways in cancer, neurological disorders, and immune regulation. For example, studies using Ab-158 have revealed MARCKS overexpression in certain tumors and its involvement in metastasis. Its phosphorylation at serine residues (e.g., Ser113/163) by PKC or other kinases modulates its membrane dissociation and functional interactions. Researchers also utilize Ab-158 to explore MARCKS' role in neuroinflammation, synaptic plasticity, and bacterial/viral pathogenesis. Validation data typically show reactivity across human, mouse, and rat samples, with applications in both basic research and drug development targeting MARCKS-mediated pathways.
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