Rabbit Polyclonal IkB-e(Ab-22) Antibody

The IκB-ε (Ab-22) antibody is a tool used to study the inhibitor of κB (IκB) family, which regulates the NF-κB signaling pathway. NF-κB is a transcription factor critical for immune response, inflammation, and cell survival, and its activity is tightly controlled by IκB proteins. IκB-ε, encoded by the NFKBIE gene, binds to NF-κB dimers (e.g., p50/RelA) in the cytoplasm, preventing their nuclear translocation and DNA binding. Unlike other IκB isoforms (e.g., IκB-α), IκB-ε has distinct structural features, including ankyrin repeat domains, and exhibits delayed degradation kinetics upon cellular stimulation, suggesting a role in modulating prolonged or secondary NF-κB activation. The IκB-ε (Ab-22) antibody specifically targets epitopes within the IκB-ε protein, enabling detection via techniques like Western blotting, immunofluorescence, or immunoprecipitation. It is widely used to investigate NF-κB regulation in contexts such as inflammation, cancer, and immune disorders. Studies employing this antibody have clarified IκB-ε’s involvement in feedback loops that fine-tune NF-κB dynamics, particularly in response to cytokines (e.g., TNF-α, IL-1) or pathogen-associated signals. Its specificity and reliability make it valuable for dissecting complex regulatory mechanisms in both physiological and disease models. Commercial sources often provide validation data, including knockout controls, to ensure minimal cross-reactivity with other IκB family members.