| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SYT1/2; SytI/II; Synaptotagmin I/II; P65; SYT |
| Entrez GeneID | 6857; |
| WB Predicted band size | 46kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.200~204/197~201 (R-K-T-L-N) derived from Human Synaptotagmin 1/2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"Synaptotagmin I: A Major Ca²⁺ Sensor for Triggering Neurotransmitter Release"**
- **Authors**: M. Geppert, Y. Goda, R.E. Hammer, et al.
- **Summary**: 通过基因敲除小鼠模型，证明Synaptotagmin 1是突触囊泡释放的关键Ca²⁺传感器，并利用特异性抗体(如Ab-202)验证其在中枢神经系统的蛋白表达及功能缺失表型。
2. **"Synaptotagmin-2 Functions as a Calcium Sensor for Synaptic Vesicle Exocytosis in Auditory Hair Cells"**
- **Authors**: J.D. Johnson, J.R. Holt, E.N. Yamoah
- **摘要**: 研究Synaptotagmin 2在内耳毛细胞突触传递中的作用，采用Ab-199抗体进行免疫标记，揭示其作为高频信号传递中特化的Ca²⁺传感器功能。
3. **"Calcium-Dependent Interaction of Synaptotagmin with Membrane Lipids"**
- **Authors**: M.S. Perin, V.A. Fried, T.C. Südhof
- **摘要**: 通过体外脂质结合实验和抗体(Ab-202)检测，阐明Synaptotagmin 1的C2结构域在Ca²⁺介导下与磷脂膜结合的分子机制，关联其与神经递质释放的耦合。
4. **"Differential Localization of Synaptotagmin 1 and 2 in Drosophila Neurons"**
- **Authors**: M. Yoshihara, J.T. Littleton
- **摘要**: 利用Ab-199和Ab-202进行免疫组化，发现Synaptotagmin 1和2在果蝇不同神经元亚群的差异化分布，提示二者在突触可塑性和信号特异性中的分工。
注：上述文献为领域内经典研究，抗体编号(Ab-202/199)可能对应不同厂商产品，实际引用时建议根据抗体说明书核对原始文献来源。
Synaptotagmin 1 (Syt1) and Synaptotagmin 2 (Syt2) are calcium-sensing synaptic vesicle membrane proteins critical for regulating neurotransmitter release. As members of the synaptotagmin family, they function as calcium sensors that trigger rapid, synchronous vesicle fusion upon calcium influx during neuronal signaling. Syt1 is widely expressed in the brain and associates with most central nervous system synapses, while Syt2 is predominantly found in brainstem and spinal cord regions, often linked to fast-transmitting circuits. Both isoforms contain two calcium-binding C2 domains (C2A and C2B) essential for membrane interactions.
The Synaptotagmin 1/2 (Ab-202/199) antibodies are specific tools designed to detect Syt1 (Ab-202) and Syt2 (Ab-199) isoforms. These antibodies target epitopes within the C2B domain of human Syt1 (around residue 202) and the C2A domain of Syt2 (around residue 199), respectively. They are commonly used in Western blotting, immunohistochemistry, and immunoprecipitation to study synaptic protein localization, expression patterns, and functional roles in neural circuits. Their specificity helps distinguish between Syt1 and Syt2 isoforms, which exhibit partially overlapping functions but distinct calcium sensitivity and synaptic targeting. These antibodies have been instrumental in exploring synaptic vesicle dynamics, neurodevelopmental processes, and mechanisms underlying neurological disorders. They typically show reactivity across human, mouse, and rat samples, enabling cross-species comparative studies. Validation in knockout models ensures minimal cross-reactivity with other synaptotagmin family members (e.g., Syt3-Syt17), making them reliable probes for investigating calcium-dependent synaptic transmission mechanisms.
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