Rabbit Polyclonal NFkB-p100(Ab-872) Antibody

The NF-κB-p100 (Ab-872) antibody is a specific reagent designed to detect the p100 protein, a precursor of the NF-κB p52 subunit encoded by the *NFKB2* gene. NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a family of transcription factors critical for regulating immune responses, inflammation, cell survival, and proliferation. The p100 protein is a key component of the non-canonical NF-κB signaling pathway, which is activated by stimuli such as CD40 ligand, lymphotoxin-β, and BAFF. In this pathway, p100 undergoes partial proteasomal processing to generate the mature p52 subunit, enabling its nuclear translocation and interaction with RelB to regulate target gene expression. The Ab-872 antibody specifically recognizes phosphorylated p100 at serine 872 (Ser872), a modification linked to its processing into p52. This phosphorylation event is mediated by IKKα (inhibitor of nuclear factor kappa-B kinase subunit alpha) during pathway activation. Researchers use this antibody in applications like Western blotting and immunofluorescence to study p100/p52 dynamics in conditions involving immune dysregulation, cancer, or inflammatory diseases. It is validated for use in human, mouse, and rat samples, providing a tool to dissect the role of non-canonical NF-κB signaling in physiological and pathological contexts. Proper experimental controls are recommended to confirm specificity.