| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CX43; CXA1; CXN-43; Connexin 43; GJA1 |
| Entrez GeneID | 2697; |
| WB Predicted band size | 43kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa. 366~370 (R-A-S-S-R) derived from Human Connexin 43. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Connexin 43 (Cx43) 抗体(Ab-368)的3篇参考文献示例(注：文献为虚拟示例，实际引用需核实)：
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1. **文献名称**: *"Connexin 43 modulates cardiac repair through gap junction communication"*
**作者**: Smith, J. et al.
**摘要**: 研究利用Cx43 (Ab-368) 抗体通过免疫组化和Western blot分析心肌梗死模型中的Cx43表达变化，发现Cx43的缺失延缓心肌细胞再生，提示其在心脏修复中的关键作用。
2. **文献名称**: *"Role of Cx43 in astrocyte-mediated neuroprotection after ischemic stroke"*
**作者**: Lee, H. & Kim, S.
**摘要**: 使用Cx43 (Ab-368) 抗体进行免疫荧光染色，证实脑缺血后星形胶质细胞中Cx43表达上调，并通过缝隙连接介导神经保护信号传递。
3. **文献名称**: *"Phosphorylation-dependent regulation of Connexin 43 in breast cancer metastasis"*
**作者**: Rodriguez, M. et al.
**摘要**: 通过Cx43 (Ab-368) 抗体检测乳腺癌细胞中Cx43的磷酸化状态，发现其与细胞迁移能力负相关，为靶向Cx43的癌症治疗提供依据。
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**提示**：实际研究中，Ab-368抗体可能对应不同厂商(如Abcam或CST)，建议通过抗体说明书或厂商官网获取准确引用文献。
Connexin 43 (Cx43), encoded by the *GJA1* gene, is a transmembrane gap junction protein critical for intercellular communication. It forms hexameric channels (connexons) that facilitate the exchange of ions, metabolites, and signaling molecules between adjacent cells, playing vital roles in tissue homeostasis, electrical coupling in cardiac muscle, and wound healing. Cx43 is dynamically regulated by post-translational modifications, including phosphorylation at specific residues like Ser368. which modulates channel gating, internalization, and interactions with signaling partners. Dysregulation of Cx43 expression or function is implicated in arrhythmias, fibrosis, cancer progression, and inflammatory disorders.
The Connexin 43 (Ab-368) antibody is a widely used tool targeting phosphorylated Ser368 (pS368), a key regulatory site linked to channel closure and protein turnover. This antibody enables researchers to investigate Cx43 activity in response to stimuli (e.g., growth factors, mechanical stress) or pathological conditions. Its applications span Western blotting, immunofluorescence, and immunohistochemistry, aiding studies in cardiomyocyte signaling, tumor microenvironment crosstalk, and tissue regeneration. By detecting pS368. Ab-368 helps elucidate mechanisms underlying cell-cell communication defects in diseases, offering insights for therapeutic strategies targeting gap junction dynamics.
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