WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/50-1/100 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Extracellular signal-regulated kinase 1 |
Entrez GeneID | 5595; |
WB Predicted band size | 42 44 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat |
Immunogen | Peptide sequence around aa. 200~204 (F-L-T-E-Y) derived from Human p44/42 MAP Kinase. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"Role of p44/42 MAPK in Cell Proliferation and Apoptosis"**
- **作者**: Johnson GL, Lapadat R.
- **摘要**: 该研究使用p44/42 MAPK (Ab-202)抗体通过Western blot和免疫荧光分析,揭示了MAPK通路在调控细胞周期进展和凋亡中的关键作用,特别是在生长因子刺激下的激活机制。
2. **"ERK1/2 Activation in Neuronal Differentiation"**
- **作者**: Chen Z, et al.
- **摘要**: 文章利用Ab-202抗体检测神经元分化模型中ERK1/2(p44/42 MAPK)的磷酸化水平,证实其在神经营养因子诱导的轴突生长和突触形成中的必要性。
3. **"p44/42 MAP Kinase in Cancer Metastasis"**
- **作者**: Roberts PJ, Der CJ.
- **摘要**: 通过免疫组化(Ab-202抗体)和功能实验,研究发现MAPK信号在肿瘤侵袭转移中的双重角色,高活性ERK1/2与特定癌症患者的预后不良相关。
4. **"Mechanical Stress Activates MAPK in Cardiac Cells"**
- **作者**: Heidkamp MC, et al.
- **摘要**: 研究使用Ab-202抗体验证心肌细胞中机械应力诱导的p44/42 MAPK瞬时激活,并探讨其与心脏肥大病理过程的联系。
(注:上述文献为示例,实际引用需根据具体研究补充。若需真实文献,建议通过PubMed或抗体供应商官网的引用列表查询。)
The p44/42 MAP Kinase (Ab-202) antibody is a widely used tool in molecular biology to detect and study the activation status of extracellular signal-regulated kinases 1 and 2 (ERK1/2), key components of the Ras-Raf-MEK-ERK signaling pathway. These serine/threonine kinases (p44 MAPK/ERK1 and p42 MAPK/ERK2) are critical for regulating cellular processes such as proliferation, differentiation, survival, and apoptosis. The antibody specifically recognizes the dual phosphorylated forms of ERK1 (Thr202/Tyr204) and ERK2 (Thr185/Tyr187), which are indicative of their activated state following upstream stimuli (e.g., growth factors, cytokines, or stress).
Developed for applications like Western blotting, immunofluorescence, and immunohistochemistry, the Ab-202 antibody helps researchers assess pathway activation in diverse contexts, including cancer, developmental biology, and drug response studies. Its specificity for the phosphorylated epitopes makes it valuable for distinguishing active ERK1/2 from inactive forms, providing insights into dynamic signaling events. Validation typically includes testing across species (human, mouse, rat) and cell lines to ensure cross-reactivity and reliability. This antibody has become a staple in studying MAPK-driven mechanisms, particularly in diseases where ERK1/2 dysregulation is implicated, such as malignancies and inflammatory disorders.
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