| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | JNK2 |
| Entrez GeneID | 5601; |
| WB Predicted band size | 46 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.181~185 (M-M-T-P-Y) derived from Human SAPK/JNK. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SAPK/JNK (Ab-183) 抗体的3篇参考文献的模拟示例(注：具体文献需根据实际数据库检索验证，以下为概括性示例)：
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1. **文献名称**: *JNK1-mediated phosphorylation of Bcl-2 regulates starvation-induced autophagy*
**作者**: Wei Y. et al.
**摘要**: 本研究使用SAPK/JNK (Ab-183) 抗体通过免疫印迹法检测JNK1在营养缺失条件下的激活状态，揭示了JNK1通过磷酸化Bcl-2调控自噬的分子机制。
2. **文献名称**: *Role of JNK signaling in oxidative stress-induced apoptosis in neuronal cells*
**作者**: Davis R.J. et al.
**摘要**: 作者利用SAPK/JNK (Ab-183) 抗体进行免疫荧光染色，证明氧化应激通过激活JNK通路诱导神经元细胞凋亡，并阐明了c-Jun磷酸化的关键作用。
3. **文献名称**: *Targeting JNK for therapeutic intervention in rheumatoid arthritis*
**作者**: Han Z. et al.
**摘要**: 通过SAPK/JNK (Ab-183) 抗体的Western blot分析，研究发现类风湿性关节炎模型中JNK活性显著升高，抑制JNK可缓解炎症反应，提示其作为治疗靶点的潜力。
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**备注**：
- 若需真实文献，建议在PubMed或Web of Science中检索关键词“SAPK/JNK Ab-183 antibody”或结合抗体生产商(如Santa Cruz产品编号sc-7345)筛选文献。
- 部分研究可能未明确标注抗体编号(Ab-183)，需结合抗体特异性描述或补充实验方法细节确认。
The SAPK/JNK (Stress-Activated Protein Kinase/c-Jun N-terminal Kinase) pathway is a critical signaling cascade within the MAPK (Mitogen-Activated Protein Kinase) family, primarily activated by cellular stressors such as UV radiation, cytokines, or oxidative damage. The JNK family comprises three isoforms (JNK1. JNK2. JNK3) encoded by distinct genes, with alternative splicing producing multiple variants. These kinases regulate diverse cellular processes, including apoptosis, inflammation, and differentiation, by phosphorylating substrates like c-Jun, a component of the AP-1 transcription factor complex.
The SAPK/JNK(Ab-183) antibody specifically detects JNK proteins phosphorylated at the Thr183/Tyr185 residues within the activation loop, a hallmark of JNK enzymatic activity. This phosphorylation event is essential for kinase activation and subsequent substrate interaction. Researchers widely use this antibody in techniques like Western blotting and immunohistochemistry to study JNK activation dynamics in response to stress signals or pathological conditions.
As JNK dysregulation is implicated in cancer, neurodegenerative diseases, and immune disorders, the Ab-183 antibody serves as a vital tool for investigating disease mechanisms and therapeutic targets. Its specificity for activated JNK, without cross-reactivity toward other MAPKs (e.g., ERK, p38), ensures reliable detection in complex biological samples, including cell lysates and tissue sections. Proper controls, such as using unstimulated samples or phosphorylation-blocking peptides, are recommended to validate results.
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