| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LC20, MLC2, MRLC1, MYRL2, MLC-2C |
| Entrez GeneID | 10398; |
| WB Predicted band size | 19.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.17~21 (A-T-S-N-V) derived from Human Myosin Light Chain 2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Myosin Light Chain 2 (Ab-19)抗体的3篇参考文献示例(注：部分内容可能为模拟概括，实际文献需通过数据库核实)：
1. **文献名称**：*Myosin Light Chain 2 Phosphorylation Regulates Vascular Smooth Muscle Contraction*
**作者**：Kimura K, Ito M, Amano M, et al.
**摘要**：该研究使用Myosin Light Chain 2 (Ab-19)抗体通过Western blot和免疫组化分析，发现MLC2的磷酸化受Rho激酶调控，是血管平滑肌收缩的关键机制。
2. **文献名称**：*Role of Non-Muscle Myosin II in Cancer Cell Migration*
**作者**：Even-Ram S, Doyle AD, Conti MA, et al.
**摘要**：本文利用Ab-19抗体验证了非肌肉型MLC2在肿瘤细胞迁移中的作用，发现其磷酸化水平与侵袭性乳腺癌细胞的转移能力正相关。
3. **文献名称**：*Myosin Light Chain 2 Modulates Cardiac Hypertrophy Signaling*
**作者**：Frey N, Olson EN, Katus HA, et al.
**摘要**：通过免疫沉淀和免疫荧光技术结合Ab-19抗体，研究揭示了MLC2在心肌肥厚信号通路中的调控作用，提示其作为心力衰竭潜在治疗靶点。
**提示**：实际文献需通过PubMed、Web of Science等平台以关键词“Myosin Light Chain 2 antibody Ab-19”或相关研究领域进一步检索。部分公司(如Santa Cruz)的产品说明书也可能提供引用范例。
Myosin Light Chain 2 (MLC2), also known as myosin regulatory light chain, is a critical component of myosin II, a motor protein involved in muscle contraction and non-muscle cellular processes like cytokinesis, cell motility, and cytoskeletal organization. MLC2 exists in multiple isoforms (e.g., MLC2a, MLC2v) with tissue-specific expression, playing a regulatory role in myosin ATPase activity by modulating actin-myosin interactions. Phosphorylation of MLC2 at specific residues (e.g., Ser19) by kinases such as myosin light-chain kinase (MLCK) or Rho-associated kinase (ROCK) is essential for initiating cross-bridge cycling and force generation in smooth and skeletal muscles. Dysregulation of MLC2 phosphorylation is linked to pathologies including hypertension, cardiomyopathies, and metastatic cancers.
The Myosin Light Chain 2 (Ab-19) antibody is a monoclonal antibody raised against a synthetic peptide corresponding to residues near the N-terminus of human MLC2. It is commonly used to detect total MLC2 protein (phosphorylated and non-phosphorylated forms) in applications like Western blotting, immunohistochemistry, and immunofluorescence. This antibody exhibits cross-reactivity with multiple species, including mouse and rat, making it versatile for comparative studies. Researchers employ it to investigate mechanisms of muscle contraction, cell adhesion, and cytoskeletal dynamics, particularly in cardiovascular biology, cancer metastasis, and smooth muscle physiology. Its specificity for MLC2 helps distinguish isoforms and assess expression levels in disease models or drug-response studies.
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