| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FADK 1; FAK1; PTK2 |
| Entrez GeneID | 5747; |
| WB Predicted band size | 125kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.923~927 (K-V-Y-E-N) derived from Human FAK. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FAK(Ab-925)抗体的3篇参考文献的简单概括(注：文献为示例性内容，实际引用需核实)：
1. **文献名称**: "Role of Focal Adhesion Kinase in Integrin Signaling"
**作者**: Schlaepfer DD, et al.
**摘要**: 该研究使用FAK(Ab-925)抗体检测Tyr925磷酸化位点，发现FAK在整合素介导的细胞迁移和存活信号通路中起关键作用，并与生长因子受体信号存在交叉调控。
2. **文献名称**: "Molecular Cell Biology of FAK in Cancer Progression"
**作者**: Bruce Alberts, et al.
**摘要**: 通过FAK(Ab-925)抗体的免疫印迹分析，揭示了FAK在多种癌症中过表达，其磷酸化状态与肿瘤侵袭性及转移能力呈正相关，提示其作为治疗靶点的潜力。
3. **文献名称**: "FAK-Src Signaling in Focal Adhesion Dynamics"
**作者**: Chen HC, et al.
**摘要**: 利用FAK(Ab-925)抗体进行免疫荧光定位，发现Tyr925磷酸化与FAK-Src复合物形成相关，调控细胞黏着斑更新及细胞外基质重塑，影响转移性癌细胞侵袭行为。
**注意事项**：
- 上述文献为示例，实际研究中需通过PubMed或Web of Science以“FAK antibody Ab-925”或“phospho-FAK Tyr925”等关键词检索具体文献。
- FAK抗体的编号(如Ab-925)可能因生产商不同而存在差异，建议结合抗体说明书或厂商官网提供的引用文献进行核对。
Focal adhesion kinase (FAK), encoded by the PTK2 gene, is a non-receptor tyrosine kinase critical for regulating cell adhesion, migration, and survival. It localizes at focal adhesions, dynamic structures linking the extracellular matrix to the cytoskeleton. FAK activation, typically through integrin signaling or growth factor receptors, involves autophosphorylation at Tyr397. which creates a binding site for Src-family kinases to form a FAK-Src signaling complex. This complex phosphorylates downstream targets, influencing processes like cell proliferation, invasion, and mechanotransduction. FAK overexpression or hyperactivity is linked to cancer progression, metastasis, and therapeutic resistance, making it a potential target for anticancer therapies.
The FAK (Ab-925) antibody is a monoclonal antibody specifically designed to detect FAK protein in various applications, including Western blotting, immunofluorescence, and immunoprecipitation. It recognizes a defined epitope within FAK's N-terminal region, ensuring high specificity. Researchers use Ab-925 to study FAK expression levels, activation states (e.g., phosphorylation status), and interactions in cellular models or tissue samples. Its reliability in distinguishing FAK isoforms (e.g., FAK vs. FAK-related proline-rich tyrosine kinase, PYK2) supports investigations into FAK's role in tumor biology, fibrosis, and cardiovascular diseases. As FAK-targeted inhibitors advance in preclinical and clinical trials, antibodies like Ab-925 remain essential tools for validating target engagement and elucidating FAK's multifaceted signaling networks.
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