| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PKB beta; Akt-2; Protein kinase B; RAC-PK-beta; |
| Entrez GeneID | 208; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa. 472~476 (Q-F-S-Y-S) derived from Human Akt2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Akt2(Ab-474)抗体的3篇参考文献，按文献名称、作者和摘要内容概括整理：
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1. **文献名称**：*Akt2 regulates cardiac metabolism and cardiomyocyte survival*
**作者**：DeBosch B. et al.
**摘要**：研究利用Akt2(Ab-474)抗体进行Western blot分析，发现Akt2基因敲除小鼠心肌细胞中胰岛素信号通路异常，导致代谢紊乱和细胞凋亡增加，表明Akt2在心脏代谢调控中起关键作用。
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2. **文献名称**：*Selective activation of Akt2 in breast cancer cells via integrin-linked kinase*
**作者**：Persad S. et al.
**摘要**：通过Akt2(Ab-474)抗体的免疫沉淀实验，证明整合素连接激酶(ILK)特异性激活Akt2而非Akt1.促进乳腺癌细胞存活和迁移，提示Akt2作为潜在治疗靶点。
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3. **文献名称**：*Akt2 ablation in skeletal muscle improves insulin sensitivity in mice*
**作者**：Lei H. et al.
**摘要**：采用Akt2(Ab-474)抗体进行组织免疫荧光分析，发现骨骼肌中Akt2缺失的小鼠胰岛素敏感性增强，揭示Akt2在肌肉胰岛素抵抗中的调控机制。
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**备注**：上述文献中使用的Akt2(Ab-474)抗体可能来源于不同厂商(如Santa Cruz或CST)，具体实验方法需结合原文补充。若需更精准的文献，建议通过抗体厂商官网(如引用列表)或PubMed高级检索筛选。
The Akt2 (Ab-474) antibody is a tool for studying Akt2. a serine/threonine kinase in the PI3K/Akt/mTOR pathway. Akt2. part of the Akt/PKB protein family, regulates cellular processes like glucose metabolism, cell survival, and proliferation. It is activated by phosphorylation at key residues, including Ser474 in the hydrophobic motif, which is critical for its full enzymatic activity. Dysregulation of Akt2 is linked to insulin resistance, diabetes, and cancers (e.g., breast, ovarian, pancreatic), where it promotes tumor growth and metastasis by inhibiting apoptosis and enhancing cell cycle progression.
The Ab-474 antibody specifically detects Akt2 phosphorylated at Ser474. enabling researchers to assess its activation status in signaling studies. Unlike pan-Akt antibodies, Ab-474’s specificity minimizes cross-reactivity with Akt1 (Ser473) or Akt3 (Ser472), making it valuable for isoform-selective analyses. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore Akt2’s role in disease mechanisms or therapeutic responses. Validated in peer-reviewed studies, this antibody aids in deciphering Akt2’s contribution to oncogenesis, metabolic disorders, and resistance to targeted therapies, supporting drug development efforts targeting the PI3K/Akt pathway.
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