Rabbit Polyclonal cdc25C(Ab-216) Antibody

The CDC25C(Ab-216) antibody is a specific tool for detecting CDC25C, a dual-specificity phosphatase critical for regulating cell cycle progression. CDC25C belongs to the CDC25 family (CDC25A, B, C) and primarily controls the G2/M phase transition by dephosphorylating and activating cyclin-dependent kinase 1 (CDK1)/cyclin B complexes. The protein contains an N-terminal regulatory domain with phosphorylation sites and a C-terminal catalytic domain. CDC25C activity is tightly regulated by DNA damage checkpoints; phosphorylation at Ser-216 (a key site recognized by some CDC25C antibodies) promotes binding to 14-3-3 proteins, sequestering it in the cytoplasm and delaying mitotic entry. The CDC25C(Ab-216) antibody, often raised against a peptide containing phosphorylated Ser-216. is widely used in studies exploring cell cycle checkpoints, DNA damage response, and cancer biology. It helps detect endogenous CDC25C phosphorylation status via techniques like Western blotting, immunofluorescence, or immunoprecipitation. Researchers employ this antibody to investigate how CDC25C dysfunction contributes to genomic instability, tumorigenesis, or chemotherapeutic resistance. Its specificity is typically validated in knockout controls or phosphorylation-sensitive assays. Cross-reactivity may vary by species, with human, mouse, and rat being common targets. Proper experimental optimization is advised due to dynamic phosphorylation changes during cell cycle progression or stress responses.