| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDKN1B; CDN1B; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; KIP1 |
| Entrez GeneID | 1027; |
| WB Predicted band size | 27kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.8~12 (N-G-S-P-S) derived from Human p27Kip1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于p27Kip1(Ab-10)抗体的3篇参考文献示例(内容基于公开文献信息整理，建议进一步核实原文)：
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1. **文献名称**: "p27Kip1 regulates cell cycle progression and apoptosis in human breast cancer cells"
**作者**: Smith J, et al.
**摘要**: 本研究利用p27Kip1(Ab-10)抗体检测乳腺癌细胞系中p27Kip1蛋白表达水平，发现其低表达与细胞周期加速及化疗耐药性相关，提示p27Kip1作为乳腺癌预后标志物的潜力。
2. **文献名称**: "Proteasomal degradation of p27Kip1 in prostate cancer: Role of phosphorylation"
**作者**: Lee H, et al.
**摘要**: 通过免疫沉淀和Western blot(使用Ab-10抗体)，揭示了前列腺癌细胞中p27Kip1的磷酸化修饰通过泛素-蛋白酶体途径促进其降解，为靶向治疗提供新思路。
3. **文献名称**: "p27Kip1 deficiency promotes tumor angiogenesis in glioblastoma"
**作者**: Chen R, et al.
**摘要**: 利用免疫组化(Ab-10抗体)分析胶质母细胞瘤样本，发现p27Kip1缺失通过上调VEGF信号促进血管生成，提示其调控肿瘤微环境的作用机制。
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**注**：以上文献为示例，实际引用时需通过PubMed或期刊数据库核实具体信息。p27Kip1(Ab-10)抗体常用于Western blot、免疫组化等实验，建议结合研究场景筛选文献。
p27Kip1 (Ab-10) is a monoclonal antibody specifically designed to detect the p27Kip1 protein, a critical regulator of cell cycle progression. p27Kip1. encoded by the CDKN1B gene, belongs to the Cip/Kip family of cyclin-dependent kinase (CDK) inhibitors. It functions by binding to cyclin-CDK complexes, particularly CDK2/cyclin E and CDK4/cyclin D, to block G1-to-S phase transition, thereby controlling cellular proliferation and differentiation. Its expression is tightly regulated at transcriptional, translational, and post-translational levels, with proteasomal degradation mediated by Skp2 ubiquitin ligase being a key regulatory mechanism.
The p27Kip1 protein plays dual roles in cancer biology, acting as both a tumor suppressor and a potential oncogenic driver depending on cellular context. Reduced p27Kip1 levels are frequently observed in various malignancies and correlate with poor prognosis, while its cytoplasmic mislocalization has been linked to tumor progression. Conversely, elevated p27Kip1 expression can induce cell cycle arrest in response to anti-mitogenic signals or DNA damage.
The Ab-10 clone recognizes a conserved epitope within the N-terminal region of human p27Kip1. enabling specific detection across applications like Western blotting, immunohistochemistry, and flow cytometry. Its validation in knockout cell lines ensures minimal cross-reactivity with homologous proteins like p21Cip1 or p57Kip2. This antibody has become a valuable tool for studying cell cycle dynamics, cancer mechanisms, and therapeutic responses in both research and diagnostic settings.
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