关键信息
-
基因名
KCNF1
- 应用
-
别名
KCNF1; Potassium voltage-gated channel subfamily F member 1; Voltage-gated potassium channel subunit Kv5.1; kH1
-
种属
Human
-
表达系统
E. coli
-
标签
GST-tag at N-terminal
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q9H3M0
-
表达区间
1-494aa
-
氨基酸序列
MDGSGERSLPEPGSQSSAASDDIEIVVNVGGVRQVLYGDLLSQYPETRLAELINCLAGGYDTIFSLCDDYDPGKREFYFDRDPDAFKCVIEVYYFGEVHMKKGICPICFKNEMDFWKVDLKFLDDCCKSHLSEKREELEEIARRVQLILDDLGVDAAEGRWRRCQKCVWKFLEKPESSCPARVVAVLSFLLILVSSVVMCMGTIPELQVLDAEGNRVEHPTLENVETACIGWFTLEYLLRLFSSPNKLHFALSFMNIVDVLAILPFYVSLTLTHLGARMMELTNVQQAVQALRIMRIARIFKLARHSSGLQTLTYALKRSFKELGLLLMYLAVGIFVFSALGYTMEQSHPETLFKSIPQSFWWAIITMTTVGYGDIYPKTTLGKLNAAISFLCGVIAIALPIHPIINNFVRYYNKQRVLETAAKHELELMELNSSSGGEGKTGGSRSDLDNLPPEPAGKEAPSCSSRLKLSHSDTFIPLLTEEKHHRTRLQSCK
-
分子量
80.08 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
KCNF1, a member of the potassium channel family, plays a critical role in regulating membrane potential and cellular excitability. Recent studies have highlighted its importance in various physiological processes, including neuronal signaling and cardiac function. Abnormalities in KCNF1 expression or function have been implicated in several diseases, such as epilepsy, cardiac arrhythmias, and other neurological disorders. The recombinant expression of KCNF1 protein is essential for studying its structure-function relationship, elucidating its mechanism of action, and understanding its role in disease. Researchers aim to produce KCNF1 in various expression systems, such as yeast, bacteria, or mammalian cells, to obtain functional and properly folded proteins. Advanced biophysical techniques, including electrophysiology and crystallography, are employed to analyze the ion channel properties and conformational dynamics of KCNF1. Moreover, understanding how KCNF1 interacts with other cellular proteins and signaling pathways is crucial for developing targeted therapeutic strategies. As interest in ion channels as drug targets grows, KCNF1's potential role in pharmacology and its contribution to the pathophysiology of relevante diseases presents an exciting area for ongoing research.
