| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CAP20; CDK-interacting protein 1; CDKN1; CDKN1A; CDN1A |
| Entrez GeneID | 1026; |
| WB Predicted band size | 21kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa.143~147 (R-Q-T-S-M) derived from Human p21Cip1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于p21Cip1(Ab-145)抗体的模拟参考文献示例，供参考：
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1. **文献名称**: "p21Cip1 Mediates Oxidative Stress-Induced Senescence via CDK Inhibition"
**作者**: Zhang, L. et al.
**摘要**: 本研究使用p21Cip1(Ab-145)抗体通过免疫印迹和免疫荧光技术，验证了氧化应激下p21在细胞衰老中的关键作用。结果显示，p21通过与CDK2结合抑制细胞周期进程，Ab-145抗体特异性识别小鼠和人类组织中的p21蛋白。
2. **文献名称**: "Antibody-Specific Detection of p21Cip1 in Colorectal Cancer Models"
**作者**: Kim, S. et al.
**摘要**: 作者利用p21Cip1(Ab-145)抗体进行免疫组化分析，发现结直肠癌组织中p21表达水平与患者预后相关。实验验证了该抗体在福尔马林固定石蜡包埋样本中的高特异性，并强调其在临床病理检测中的潜在应用。
3. **文献名称**: "Mechanistic Insights into p21-Dependent Cell Cycle Arrest"
**作者**: Harper, J.W. & Elledge, S.J.
**摘要**: 该研究通过免疫沉淀(使用Ab-145抗体)和质谱分析，揭示了p21与CDK复合物的相互作用机制，证实其在DNA损伤后调控G1期停滞的功能。抗体在识别全长p21蛋白时表现出高灵敏度。
4. **文献名称**: "Validation of p21Cip1 Antibody (Ab-145) in Murine Tissues"
**作者**: Rivera, A. et al.
**摘要**: 本文系统评估了Ab-145抗体在小鼠肝脏和肾脏组织中的特异性，通过敲除模型排除了非特异性信号。研究表明该抗体适用于流式细胞术和冰冻切片分析，为体内研究提供了可靠工具。
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**说明**：上述文献为模拟示例，实际引用时需通过PubMed、Google Scholar或抗体供应商官网(如Santa Cruz Biotechnology、Cell Signaling Technology等)检索具体信息。建议以“p21Cip1 Ab-145 antibody”为关键词，或查阅该抗体的产品说明书(Cat# sc-6246等)获取被引研究。
The p21Cip1 protein, encoded by the CDKN1A gene, is a critical cyclin-dependent kinase (CDK) inhibitor that regulates cell cycle progression by binding to cyclin-CDK complexes, particularly at the G1/S checkpoint. It is transcriptionally activated by p53 in response to DNA damage or stress, inducing cell cycle arrest to allow repair or apoptosis. Dysregulation of p21Cip1 is linked to cancer, aging, and degenerative diseases.
The p21Cip1(Ab-145) antibody is a monoclonal antibody raised against a specific epitope of human p21Cip1. often used to detect endogenous p21Cip1 levels in applications like Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF). Its specificity is validated through knockdown/knockout controls, ensuring minimal cross-reactivity with related CDK inhibitors (e.g., p27 or p57). The antibody typically recognizes a ~21 kDa band, though post-translational modifications may alter migration.
Widely utilized in cancer research, Ab-145 helps study p21Cip1's role in tumor suppression, chemotherapy response, and cellular senescence. It is also employed in exploring tissue regeneration, stem cell dynamics, and DNA damage response pathways. Proper validation under experimental conditions is recommended due to context-dependent p21Cip1 expression and potential isoform variability.
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