Rabbit Polyclonal ALKBH5 Antibody

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     All lanes : Anti-ALKBH5 Antibody (Center) at 1:500 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Overlay histogram showing U-2 OS cells stained with P34429(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34429,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

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     P34429 staining ALKBH5  in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Samples were incubated with primary antibody (1/100) for 1 hours at room temperature.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

The ALKBH5 antibody is a crucial tool for studying the ALKBH5 protein, a member of the AlkB homolog (ALKBH) family of enzymes. ALKBH5 is an Fe(II)/α-ketoglutarate-dependent dioxygenase that specifically catalyzes the demethylation of N6-methyladenosine (m6A), the most prevalent internal chemical modification in eukaryotic mRNA. Discovered in 2011. ALKBH5 regulates m6A dynamics, influencing mRNA metabolism, stability, splicing, and translation. Its role extends to diverse biological processes, including stem cell differentiation, tumorigenesis, immune response, and reproductive development. Dysregulation of ALKBH5 has been linked to cancers (e.g., glioblastoma, breast cancer), infertility, and metabolic disorders.

ALKBH5 antibodies enable the detection, localization, and functional analysis of this enzyme in cells and tissues. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). High-quality antibodies are validated for specificity, cross-reactivity (e.g., human, mouse), and application compatibility. Researchers employ these antibodies to explore ALKBH5's expression patterns, its interplay with m6A-binding proteins, and its pathophysiological roles. As m6A epigenetics gains prominence, ALKBH5 antibodies remain essential for decoding its regulatory mechanisms and therapeutic potential in diseases.