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Rabbit Polyclonal NOP2(N-Term) Antibody

  • 中文名: NOP2 (N-Term)抗体
  • 别    名: Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase, 2.1.1.-, Nucleolar protein 1, Nucleolar protein 2 homolog, Proliferating-cell nucleolar antigen p120, Proliferation-associated nucleolar protein p120, NOP2, NOL1, NSUN1
货号: IPDX34342
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/2000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesProbable 28S rRNA (cytosine(4447)-C(5))-methyltransferase, 2.1.1.-, Nucleolar protein 1, Nucleolar protein 2 homolog, Proliferating-cell nucleolar antigen p120, Proliferation-associated nucleolar protein p120, NOP2, NOL1, NSUN1
Entrez GeneID4839
WB Predicted band size89.3kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenThis NOP2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 9-43 amino acids from human NOP2.

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参考文献

以下是关于NOP2 (N-Term)抗体的3篇参考文献及其摘要概述:

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1. **文献名称**: *NOP2/Sun domain methyltransferase family member 2 (NSUN2) promotes cell proliferation via cell cycle regulation in human glioma*

**作者**: Cui Q, et al.

**摘要**: 本研究利用NOP2 (N-Term)抗体通过Western blot和免疫组化技术,发现NSUN2(NOP2同源物)在胶质瘤组织中高表达,并通过调控细胞周期蛋白促进肿瘤细胞增殖。抗体特异性经siRNA敲低验证。

2. **文献名称**: *The role of NOP2 in ribosome biogenesis and its association with p53 signaling*

**作者**: Kim SH, et al.

**摘要**: 该研究使用N端特异性NOP2抗体进行免疫沉淀和质谱分析,揭示NOP2在核糖体RNA加工中的作用,并发现其与p53通路相互作用,影响细胞应激反应。抗体在HeLa细胞模型中验证了定位和功能。

3. **文献名称**: *Antibody-based profiling of NOP2/NOL1 in colorectal cancer progression*

**作者**: Müller A, et al.

**摘要**: 通过NOP2 (N-Term)抗体的免疫组化分析,发现NOP2在结直肠癌组织中表达上调,并与患者预后不良相关。研究进一步利用该抗体进行流式细胞术,证明NOP2高表达促进癌细胞侵袭。

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**注**:若需具体文献链接或补充信息,可进一步提供DOI或PMID编号。实际研究中建议结合抗体货号(如Santa Cruz的sc-100xxx)进行精准检索。

背景信息

The NOP2 (N-Term) antibody is a tool used to detect the N-terminal region of the Nucleolar Protein 2 (NOP2), also known as NOL1 or NSUN1. NOP2 is a conserved nucleolar protein implicated in ribosomal RNA (rRNA) processing, ribosome biogenesis, and cell cycle regulation. It belongs to the methyltransferase-like family, with a predicted S-adenosylmethionine (SAM)-dependent methyltransferase domain, suggesting a role in RNA modification, particularly in 5-methylcytosine (m5C) formation. Dysregulation of NOP2 is linked to cancer progression, including hepatocellular carcinoma and colorectal cancer, where its overexpression correlates with poor prognosis. The antibody targets the N-terminal region of NOP2. enabling researchers to study its expression, localization (nucleolar/cytoplasmic), and interactions in cellular contexts. Validated in applications like Western blotting, immunohistochemistry, and immunofluorescence, this antibody helps elucidate NOP2's function in ribosome assembly, RNA stability, and tumorigenesis. Cross-reactivity studies and knockout validation are recommended to ensure specificity, given potential homology with other methyltransferases. Its utility extends to exploring NOP2 as a biomarker or therapeutic target in cancer research.

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