Rabbit Polyclonal NKX2-5 Antibody

The NKX2-5 antibody is a crucial tool in studying the role of the NKX2-5 protein, a transcription factor encoded by the NKX2-5 gene. This gene, part of the NKX2 family, is essential for heart development, particularly in regulating cardiac cell differentiation, chamber formation, and conduction system maturation. NKX2-5 mutations are linked to congenital heart defects (e.g., atrial septal defects) and arrhythmias. Researchers use NKX2-5 antibodies to detect and visualize the protein’s expression in tissues, enabling studies on cardiac development, disease mechanisms, and regenerative medicine. These antibodies are typically produced in hosts like rabbits or mice, targeting specific epitopes via immunogen-derived peptides. Validation methods (e.g., Western blot, immunohistochemistry, knockout controls) ensure specificity. Applications span developmental biology, cancer research (due to NKX2-5's tumor-suppressive roles in some cancers), and stem cell studies, where NKX2-5 is a marker for cardiomyocyte differentiation. Commercial NKX2-5 antibodies vary in clonality (monoclonal/polyclonal) and conjugates (e.g., fluorescent tags), allowing flexibility in experimental design. Proper controls are critical due to potential cross-reactivity with related proteins. Overall, NKX2-5 antibodies are indispensable for unraveling cardiac biology and associated pathologies.