| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein phosphatase 1 regulatory subunit 3E, PPP1R3E |
| Entrez GeneID | 90673 |
| WB Predicted band size | 30.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This PPP1R3E antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 159-192 amino acids from the Central region of human PPP1R3E. |
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由于我无法直接访问实时数据库(如PubMed或Google Scholar)，以下为基于知识背景构造的示例文献格式。如需真实文献，建议通过学术数据库检索关键词“PPP1R3E antibody”或“PPP1R3E research”：
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1. **文献名称**: *PPP1R3E regulates hepatic glycogen synthesis through interaction with protein phosphatase 1*
**作者**: Smith A, et al.
**摘要**: 本研究利用特异性PPP1R3E抗体，通过免疫共沉淀和Western blot技术，揭示了PPP1R3E在肝脏中通过与蛋白磷酸酶1(PP1)结合调控糖原合成的分子机制，为代谢疾病研究提供新靶点。
2. **文献名称**: *Development and validation of a monoclonal antibody against human PPP1R3E for diagnostic applications*
**作者**: Zhang L, et al.
**摘要**: 报道了一种高特异性抗人PPP1R3E单克隆抗体的开发，验证其在ELISA和免疫组化中的应用，并探讨其在糖尿病组织样本中的表达差异。
3. **文献名称**: *PPP1R3E expression in skeletal muscle: Role in insulin resistance*
**作者**: Brown K, et al.
**摘要**: 通过免疫荧光和Western blot分析，发现PPP1R3E在胰岛素抵抗模型小鼠骨骼肌中的表达下调，提示其可能参与胰岛素信号通路的调控。
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**建议检索方法**：
- 使用PubMed(https://pubmed.ncbi.nlm.nih.gov)或Google Scholar，搜索关键词：
`"PPP1R3E antibody"`、`"PPP1R3E immunoblot"`、`"PPP1R3E expression"`
- 查找抗体公司(如Abcam、Thermo Fisher)的产品说明书，通常附相关引用文献。
The PPP1R3E antibody is a crucial tool for investigating the regulatory mechanisms of protein phosphatase 1 (PP1), a serine/threonine phosphatase central to diverse cellular processes. PPP1R3E (Protein Phosphatase 1 Regulatory Subunit 3E) is one of numerous regulatory subunits that dictate PP1’s substrate specificity, localization, and activity. Specifically, PPP1R3E is implicated in glycogen metabolism, where it likely facilitates PP1’s interaction with enzymes governing glycogen synthesis and breakdown, such as glycogen synthase and phosphorylase. This regulatory role positions PPP1R3E as a potential player in metabolic disorders, including diabetes and obesity.
Antibodies targeting PPP1R3E enable researchers to study its expression, subcellular distribution, and interaction networks via techniques like Western blotting, immunofluorescence, and immunoprecipitation. These antibodies are typically validated for specificity using knockout controls or siRNA-mediated knockdown to ensure minimal cross-reactivity with related PP1 regulatory subunits. Their development has advanced studies exploring tissue-specific PP1 regulation, particularly in metabolic tissues like liver, muscle, and adipose.
Current research focuses on elucidating PPP1R3E’s role in insulin signaling, glycogen storage diseases, and cancer, where dysregulated phosphorylation pathways are common. As PP1 dysfunction is linked to neurodegenerative diseases and cardiac pathologies, PPP1R3E antibodies may also contribute to broader biomedical investigations. Commercial availability of these reagents, often in polyclonal or monoclonal formats, supports both basic and translational research, underscoring their importance in cellular signaling and metabolic research.
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