| WB | 1/1000-1/8000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | 14-3-3 protein gamma-1, ywhag1, ywhag |
| Entrez GeneID | 117604 |
| WB Predicted band size | 28.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This DANRE ywhag1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-162 amino acids from the Central region of DANRE ywhag1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于斑马鱼(DANRE)Ywhag1抗体的假设性参考文献示例，基于该领域常见研究方向构建：
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1. **文献名称**：*"Ywhag1 modulates early neural development in zebrafish through 14-3-3 signaling"*
**作者**：Li, X., & Wang, Y.
**摘要**：本研究利用斑马鱼模型，通过定制兔源多克隆抗体验证Ywhag1蛋白在胚胎神经管形成中的表达。抗体通过Western blot和免疫荧光验证特异性，结果显示Ywhag1通过结合Shh信号通路调控神经前体细胞分化。
2. **文献名称**：*"Antibody-based profiling of 14-3-3γ (Ywhag1) in zebrafish oxidative stress models"*
**作者**：Garcia, R., et al.
**摘要**：开发了一种小鼠单克隆抗斑马鱼Ywhag1抗体，用于检测氧化应激下肝脏组织中Ywhag1的亚细胞定位变化。抗体特异性通过CRISPR/Cas9敲除株的阴性结果验证，证实Ywhag1在应激反应中的线粒体易位。
3. **文献名称**：*"Generation and application of a polyclonal antibody for zebrafish Ywhag1 in cardiac regeneration studies"*
**作者**：Chen, J., et al.
**摘要**：报道了一种兔多克隆抗体的制备与验证，用于检测斑马鱼心脏损伤后Ywhag1的动态表达。免疫组化显示Ywhag1在再生心肌细胞中上调，并与Notch通路蛋白共定位，提示其参与组织修复。
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**备注**：以上为模拟示例，实际文献需通过PubMed或ZFIN(斑马鱼数据库)检索。建议使用关键词“Ywhag1 Danio rerio antibody”或访问抗体供应商网站(如GeneTex、Abcam)获取引用文献。
The DANRE Ywhag1 antibody is a specific tool developed to detect the 14-3-3 gamma protein (Ywhag1) in the zebrafish (*Danio rerio*) model. The 14-3-3 protein family consists of conserved regulatory molecules involved in diverse cellular processes, including signal transduction, apoptosis, and cell cycle control. Ywhag1. encoded by the *ywhag1* gene, plays a critical role in binding phosphorylated serine/threonine residues on target proteins, modulating their activity, stability, or localization. In zebrafish research, this antibody is particularly valuable for studying developmental biology, neurogenesis, and disease mechanisms due to the model's genetic tractability and transparent embryos.
The DANRE Ywhag1 antibody is typically produced in rabbits or mice using immunogenic peptides derived from conserved regions of the zebrafish protein. It is validated for applications like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression and localization in tissues or embryos. Researchers utilize this antibody to explore Ywhag1's involvement in signaling pathways, such as PI3K/AKT or MAPK, and its interactions with disease-related proteins, including those linked to cancer or neurodegenerative disorders. Its specificity for zebrafish Ywhag1 ensures minimal cross-reactivity with other 14-3-3 isoforms, making it a reliable tool for in vivo functional studies. This antibody has contributed to understanding conserved molecular mechanisms across vertebrates and their implications in human pathologies.
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