WB | 1/1000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/100-1/500 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 1/10-1/50 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Speedy protein A, Rapid inducer of G2/M progression in oocytes A, RINGO A, hSpy/Ringo A, Speedy-1, Spy1, SPDYA (HGNC:30613) |
Entrez GeneID | 245711 |
WB Predicted band size | 36.5kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This SPDYA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the Central region of human SPDYA. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与SPDYA抗体相关的研究文献概览(注:部分信息基于领域内典型研究方向模拟整理):
1. **文献名称**: "SPDYA regulates proliferation and checkpoint activation in mammalian germ cells"
**作者**: Höög C. et al.
**摘要**: 本研究利用SPDYA特异性抗体(兔多克隆)检测小鼠睾丸组织中SPDYA蛋白表达,发现其在精母细胞减数分裂中调控细胞周期进程,并参与DNA损伤检查点响应。抗体通过免疫组化验证定位。
2. **文献名称**: "SPDYA promotes hepatocellular carcinoma progression via CDK2-mediated cell cycle dysregulation"
**作者**: Xie L. et al.
**摘要**: 通过Western blot和免疫荧光(使用小鼠抗人SPDYA单克隆抗体)分析肝癌细胞系,证实SPDYA通过激活CDK2驱动细胞周期异常,促进肿瘤增殖,抗体特异性经siRNA敲降实验验证。
3. **文献名称**: "The oncogenic role of SPDYA in osteosarcoma through mTOR signaling pathway"
**作者**: Chen Y. et al.
**摘要**: 使用兔源SPDYA抗体(货号abXXXXX)进行免疫沉淀实验,发现SPDYA与mTOR通路蛋白互作,促进骨肉瘤细胞增殖。研究通过临床样本的免疫组化显示SPDYA高表达与患者预后不良相关。
**注**:以上为模拟文献,实际引用时请核实PubMed/SCI数据库,部分抗体货号需参考具体厂商(如Abcam、CST等)的验证数据。
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