| WB | 1/1000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Cyclic AMP-dependent transcription factor ATF-4, cAMP-dependent transcription factor ATF-4, Activating transcription factor 4, atf4 {ECO:0000312|ZFIN:ZDB-GENE-040426-2340}, atf4b1 |
| Entrez GeneID | 406514 |
| WB Predicted band size | 36.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This DANRE atf4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-314 amino acids from the C-terminal region of DANRE atf4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于斑马鱼(Danio rerio)ATF4抗体的假设性参考文献示例(注：部分文献信息为示例性模拟，实际引用时需核实真实来源)：
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1. **文献名称**：*ATF4 Mediates Oxidative Stress Response in Zebrafish Embryos*
**作者**：Zhang Y, Li Q, Wang H.
**摘要**：本研究利用特异性抗ATF4抗体，通过免疫印迹和免疫荧光技术，揭示了斑马鱼胚胎在氧化应激条件下ATF4蛋白的核转位及下游基因调控机制，证实其在内质网应激通路中的关键作用。
2. **文献名称**：*Generation and Application of a Polyclonal Antibody for Detecting ATF4 in Danio rerio Tissues*
**作者**：Müller S, et al.
**摘要**：报道了一种针对斑马鱼ATF4蛋白的多克隆抗体的开发与验证，通过免疫组化及Western blot分析，证明该抗体在成年斑马鱼肝脏和脑组织中的高特异性，为研究ATF4的生理功能提供了工具。
3. **文献名称**：*ATF4 Knockdown Disrupts Skeletal Development in Zebrafish*
**作者**：Garcia-Reyes M, et al.
**摘要**：利用ATF4抗体进行表型分析，发现ATF4基因敲低导致斑马鱼幼体骨骼发育异常，表明ATF4通过调控成骨相关基因(如BMP2)在骨骼形成中起重要作用。
4. **文献名称**：*Cross-Species Comparison of ATF4 Expression under Hypoxic Conditions*
**作者**：Tanaka K, et al.
**摘要**：比较斑马鱼与哺乳动物ATF4在低氧应激中的表达差异，使用物种特异性抗体揭示斑马鱼ATF4在鳃组织中的独特激活模式，提示其进化保守性与功能分化。
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**注意**：以上文献信息为示例性内容，实际研究中请通过学术数据库(如PubMed、Web of Science)以关键词“Danio rerio ATF4 antibody”或“zebrafish ATF4 antibody”检索真实文献，并核对作者、标题及摘要准确性。
The DANRE ATF4 antibody is designed to detect the activating transcription factor 4 (ATF4) protein in *Danio rerio* (zebrafish), a widely used model organism for developmental biology and disease research. ATF4. a member of the ATF/CREB family of basic leucine zipper (bZIP) transcription factors, plays a critical role in cellular stress responses, including endoplasmic reticulum (ER) stress, amino acid deprivation, and oxidative stress. It regulates genes involved in metabolism, apoptosis, autophagy, and redox homeostasis, often through the integrated stress response (ISR) pathway.
In zebrafish studies, the DANRE ATF4 antibody enables researchers to investigate ATF4 expression and function during embryogenesis, tissue regeneration, or stress adaptation. It is commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to localize ATF4 in tissues such as the liver, brain, or developing embryos. The antibody's specificity is typically validated via knockout controls or peptide blocking assays to ensure minimal cross-reactivity.
Understanding ATF4 dynamics in zebrafish models contributes to insights into human diseases linked to stress signaling dysregulation, including neurodegenerative disorders, metabolic syndromes, and cancer. This tool thus bridges molecular mechanisms across species, aiding translational research.
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