| WB | 1/1000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Homeobox protein Hox-C9a, Hox-C9, hoxc9a, hoxc9 |
| WB Predicted band size | 29.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This DANRE hoxc9a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 234-260 amino acids from the C-terminal region of DANRE hoxc9a. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于斑马鱼(Danio rerio)**hoxc9a抗体**的3篇假设性参考文献示例，涵盖其在不同研究中的应用：
1. **文献名称**：*"Hoxc9a expression dynamics in zebrafish somitic development revealed by a novel monoclonal antibody"*
**作者**：Zhang L, et al.
**摘要**：本研究开发了一种针对斑马鱼Hoxc9a蛋白的单克隆抗体，通过免疫印迹和免疫组化验证其特异性，并揭示了hoxc9a在体节分化和脊髓区域化中的时空表达模式。
2. **文献名称**：*"Functional analysis of Hoxc9a in zebrafish motor neuron patterning using CRISPR and antibody-based targeting"*
**作者**：Nguyen T, et al.
**摘要**：结合CRISPR基因编辑和Hoxc9a抗体，研究证明hoxc9a通过调控下游靶基因参与脊髓运动神经元亚型的定位，抗体用于验证蛋白表达缺失后的表型变化。
3. **文献名称**：*"Comparative study of Hox gene antibody specificity across vertebrate models: Focus on zebrafish Hoxc9a"*
**作者**：Müller R, et al.
**摘要**：通过比较不同物种Hox蛋白抗体的交叉反应性，确认斑马鱼Hoxc9a抗体的物种特异性，并应用于胚胎发育中前-后轴模式的蛋白定位研究。
(注：以上文献为假设示例，实际引用需查询具体数据库获取真实文献。)
The DANRE hoxc9a antibody is a specialized tool used in developmental biology research to study the expression and function of the hoxc9a gene in zebrafish (*Danio rerio*). Hox genes, including hoxc9a, are critical regulators of embryonic development, particularly in establishing body segmentation and regional identity along the anterior-posterior axis. As a member of the Homeobox (Hox) gene family, hoxc9a encodes a transcription factor that guides the formation of specific anatomical structures during embryogenesis, particularly in the posterior trunk and tail regions.
This antibody is typically generated against a peptide or recombinant protein corresponding to a conserved region of the zebrafish Hoxc9a protein, enabling detection in various experimental applications such as immunohistochemistry, Western blotting, or whole-mount in situ hybridization. Researchers use it to map hoxc9a expression patterns in developing embryos, investigate its role in axial patterning, or study mutations affecting hoxc9a function.
Zebrafish are a favored model for vertebrate developmental studies due to their transparent embryos and rapid external development. The DANRE hoxc9a antibody thus supports comparative analyses of Hox gene conservation and divergence across species. Validation often includes testing in wild-type versus hoxc9a-mutant embryos to confirm specificity. Its utility extends to fields like evolutionary developmental biology (evo-devo) and studies of congenital malformations linked to Hox gene dysregulation.
×
