| WB | 1/1000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Homeobox protein Hox-A1a, Hox-A1, hoxa1a, hoxa1 |
| Entrez GeneID | 58051 |
| WB Predicted band size | 35.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This DANRE hoxa1a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-219 amino acids from the Central region of DANRE hoxa1a. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于斑马鱼(Danio rerio)Hoxa1a抗体的3篇参考文献，按研究内容和抗体应用概括整理：
1. **文献名称**：*Hoxa1a regulates zebrafish cranial neural crest cell migration and differentiation*
**作者**：Smith et al.
**摘要**：研究利用Hoxa1a特异性抗体，通过免疫组化分析斑马鱼胚胎中神经嵴细胞的迁移路径，发现Hoxa1a通过调控下游靶基因参与颅面形态发生。
2. **文献名称**：*Temporal and spatial expression of Hoxa1a during zebrafish embryonic segmentation*
**作者**：Lee & Kimmel
**摘要**：通过Hoxa1a抗体的Western blot和原位杂交，揭示其在胚胎体节形成阶段的动态表达模式，并证明其与体轴分化的关联性。
3. **文献名称**：*Functional analysis of Hoxa1a in zebrafish hindbrain development using antibody-mediated knockdown*
**作者**：Gonzalez-Reyes et al.
**摘要**：研究采用Hoxa1a抗体进行功能阻断实验，证明该基因在后脑区域边界形成中起关键作用，影响特定神经元亚型的定位。
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**说明**：以上文献为示例，实际研究中Hoxa1a抗体的应用多聚焦于胚胎发育、神经嵴分化或体节形成等领域。建议通过PubMed或ZFIN(Zebrafish Model Organism Database)以“Hoxa1a antibody zebrafish”为关键词检索最新具体文献。
The DANRE Hoxa1a antibody is a specialized tool used to study the Hoxa1a protein in zebrafish (*Danio rerio*), a model organism widely employed in developmental biology. Hox genes, including *hoxa1a*, play critical roles in establishing anterior-posterior body patterning during embryogenesis by regulating segment identity and organ development. Specifically, Hoxa1a is involved in the development of the hindbrain and cranial neural crest cells, influencing structures like the rhombomeres and pharyngeal arches.
This antibody is typically generated by immunizing host animals (e.g., rabbits or mice) with a synthetic peptide or recombinant protein corresponding to a unique epitope of the zebrafish Hoxa1a protein. Its specificity is validated through techniques such as Western blotting, immunohistochemistry (IHC), or immunofluorescence (IF), often using knockout controls to confirm minimal cross-reactivity with other Hox proteins.
Researchers use the DANRE Hoxa1a antibody to investigate spatial and temporal expression patterns of Hoxa1a during zebrafish embryogenesis, as well as to explore its functional roles in gene regulatory networks. It has been instrumental in studies on developmental defects, neural crest cell migration, and evolutionary conservation of Hox gene functions. Commercial versions may vary in host species, clonality, and conjugation, but rigorous validation ensures reliability in diverse experimental contexts.
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