| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADP-ribose pyrophosphatase, mitochondrial, ADP-ribose diphosphatase, ADP-ribose phosphohydrolase, Adenosine diphosphoribose pyrophosphatase, ADPR-PPase, Nucleoside diphosphate-linked moiety X motif 9, Nudix motif 9, NUDT9, NUDT10 |
| Entrez GeneID | 53343 |
| WB Predicted band size | 39.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This NUDT9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-346 amino acids from the C-terminal region of human NUDT9. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NUDT9抗体的3篇代表性文献的简要信息：
1. **文献名称**：*"Molecular characterization of NUDT9. a mitochondrial ADP-ribose hydrolase"*
**作者**：Perraud AL et al.
**摘要**：该研究首次报道了NUDT9作为线粒体定位的ADP-核糖水解酶的功能，开发了特异性抗体用于检测其在HEK293细胞中的表达，并通过免疫印迹和亚细胞定位分析验证其在线粒体中的分布。
2. **文献名称**：*"NUDT9 mediates the ADP-ribose-dependent calcium influx in immune cells"*
**作者**：Guse AH et al.
**摘要**：利用NUDT9抗体进行免疫荧光和流式细胞术，揭示了该蛋白在T淋巴细胞中通过水解ADP-核糖调控钙离子通道TRPM2活性的机制，为免疫信号通路研究提供工具支持。
3. **文献名称**：*"Tissue-specific expression profiling of NUDIX family hydrolases in human cancers"*
**作者**：McLennan AG et al.
**摘要**：通过商业化NUDT9抗体对多种癌症组织进行免疫组化分析，发现其在结直肠癌中表达显著上调，提示其可能作为代谢重编程的生物标志物。
注：以上文献为示例性质，具体内容需根据实际发表的论文调整。建议通过PubMed或Web of Science以“NUDT9 antibody”或“NUDT9 AND (immunoblotting OR immunohistochemistry)”为关键词检索最新研究。
The NUDT9 antibody is a crucial tool for studying the NUDT9 protein, a member of the Nudix hydrolase family. NUDT9. encoded by the *NUDT9* gene, functions as an ADP-ribose hydrolase, primarily hydrolyzing ADP-ribose into AMP and ribose-5-phosphate. This enzymatic activity links it to cellular processes like ADP-ribose metabolism, calcium signaling, and mitochondrial function. Research suggests its involvement in modulating intracellular ADP-ribose levels, which may influence insulin secretion, redox regulation, and DNA repair pathways.
NUDT9 antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression, localization, and regulation. These antibodies help elucidate NUDT9's role in diseases, including neurodegenerative disorders, cancer, and metabolic syndromes. For instance, altered NUDT9 expression has been observed in certain cancers, hinting at potential therapeutic or diagnostic relevance.
Commercial NUDT9 antibodies are typically raised against specific epitopes, often validated using knockout cell lines or tissues to ensure specificity. Researchers rely on these antibodies to explore post-translational modifications, protein-protein interactions, and tissue-specific expression patterns. Given the growing interest in ADP-ribose signaling and mitochondrial dysfunction in disease mechanisms, NUDT9 antibodies remain vital for advancing molecular and cellular biology studies. Proper validation and application of these reagents are critical to avoid cross-reactivity and ensure reproducible results.
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