| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Autophagy-related protein 16-2, APG16-like 2, WD repeat-containing protein 80, ATG16L2, WDR80 |
| Entrez GeneID | 89849 |
| WB Predicted band size | 69.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ATG16L2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-304 amino acids from the Central region of human ATG16L2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ATG16L2(Center K292)抗体的部分参考文献示例(内容为虚构，仅供参考)：
1. **"ATG16L2 modulates autophagosome formation in intestinal epithelial cells"**
- **作者**: Smith J, et al.
- **摘要**: 本研究利用针对ATG16L2中心区域K292的特异性抗体，揭示了该蛋白在肠道上皮细胞自噬体形成中的关键作用，并发现其与克罗恩病风险的关联。
2. **"Role of ATG16L2 phosphorylation in cancer cell survival"**
- **作者**: Lee H, et al.
- **摘要**: 通过K292位点抗体检测ATG16L2的磷酸化状态，发现其在肺癌细胞中通过调控线粒体自噬影响化疗耐药性，为靶向治疗提供新思路。
3. **"Characterization of ATG16L2 isoforms in neurodegenerative disorders"**
- **作者**: Garcia R, et al.
- **摘要**: 使用K292中心区域抗体鉴定了ATG16L2的不同剪切变体，发现其异常表达与阿尔茨海默病中tau蛋白聚集的相关性。
4. **"ATG16L2 interacts with NLRP3 inflammasome in macrophage polarization"**
- **作者**: Chen L, et al.
- **摘要**: 通过免疫共沉淀(使用抗K292抗体)证实ATG16L2与NLRP3炎症小体的相互作用，阐明其在巨噬细胞M1/M2极化中的调控机制。
**备注**：上述文献为示例，实际研究中建议通过PubMed或Google Scholar以“ATG16L2 antibody K292”为关键词检索，或查阅抗体供应商(如CST、Abcam)官网的产品引用文献列表。部分研究可能未明确标注抗体表位信息，需结合实验方法细节判断。
The ATG16L2 (Center K292) antibody is designed to target a specific region within the ATG16-like 2 (ATG16L2) protein, a member of the autophagy-related 16 (ATG16) family. ATG16L2 plays a role in the autophagy pathway, a critical cellular process for degrading damaged organelles and proteins. While its paralog, ATG16L1. is well-characterized in canonical autophagy, ATG16L2 is implicated in non-canonical autophagy and other cellular functions, including membrane trafficking and immune responses. The antibody specifically recognizes a central epitope surrounding lysine 292 (K292), a residue potentially involved in protein-protein interactions or post-translational modifications.
This antibody is commonly used in research to study ATG16L2 expression, localization, and function in various tissues and disease models, such as cancer, neurodegenerative disorders, and inflammatory conditions. It is validated for applications like Western blotting, immunofluorescence, and immunoprecipitation, often helping to elucidate ATG16L2's regulatory mechanisms in autophagy-independent pathways. Researchers also utilize it to explore genetic variants or dysregulation linked to diseases, as ATG16L2 polymorphisms have been associated with susceptibility to certain autoimmune and metabolic disorders. Specificity for the K292 region ensures minimal cross-reactivity with homologous proteins like ATG16L1. making it a valuable tool for dissecting the unique roles of ATG16L2 in cellular homeostasis and disease pathogenesis.
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