Rabbit Polyclonal TRIM28(N-term) Antibody

TRIM28 (N-terminal) antibody is a research tool designed to target the amino-terminal region of TRIM28 (Tripartite Motif-Containing Protein 28), also known as KAP1 or TIF1β. TRIM28 is a multifunctional scaffolding protein belonging to the TRIM family, which plays a critical role in epigenetic regulation, chromatin remodeling, and transcriptional repression. It acts as a co-repressor by recruiting histone methyltransferases (e.g., SETDB1), histone deacetylases (HDACs), and heterochromatin protein 1 (HP1) to establish transcriptionally silent chromatin states. The N-terminal region of TRIM28 contains conserved domains, including the RBCC (RING, B-box, coiled-coil) motif, which mediates protein-protein interactions, oligomerization, and E3 ubiquitin ligase activity. Antibodies targeting this region are widely used to detect full-length TRIM28 in applications such as Western blotting, immunoprecipitation, and immunofluorescence. They are particularly valuable in studies exploring TRIM28's roles in embryonic development, genomic imprinting, DNA damage response, and viral defense. TRIM28 dysregulation has been linked to cancers, neurodevelopmental disorders, and stem cell pluripotency, making this antibody a key reagent in biomedical research. Validation of specificity, often through knockout cell lines or siRNA knockdown, is essential to ensure accurate detection of the ~110 kDa protein across human, mouse, and rat samples.