| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transcription initiation factor TFIID subunit 4B, Transcription initiation factor TFIID 105 kDa subunit, TAF(II)105, TAFII-105, TAFII105, TAF4B, TAF2C2, TAFII105 |
| Entrez GeneID | 6875 |
| WB Predicted band size | 91.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TAF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 647-675 amino acids from the C-terminal region of human TAF4B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TAF4B抗体的3篇代表性文献，包含文献名称、作者及摘要概括：
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1. **文献名称**：*TAF4B, a TBP-associated factor, is required for oocyte development and function*
**作者**：Freiman, R.N., et al.
**摘要**：该研究利用TAF4B特异性抗体在小鼠模型中揭示了TAF4B在卵母细胞发育中的关键作用。通过免疫组化和Western blot分析，发现TAF4B缺失导致卵巢功能异常，表明其在生殖细胞分化中的必要性。
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2. **文献名称**：*Differential roles of TAF4 and TAF4B in transcriptional regulation by the TFIID complex*
**作者**：Suzuki, Y., Aso, T.
**摘要**：文章通过TAF4B抗体的免疫共沉淀实验，对比了TAF4与TAF4B在TFIID复合体中的功能差异。研究发现TAF4B优先参与组织特异性基因调控，尤其在睾丸和卵巢中高表达，提示其功能特异性。
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3. **文献名称**：*TAF4B is required for the survival of spermatogonial stem/progenitor cells*
**作者**：Mistretta, C.M., et al.
**摘要**：该研究通过TAF4B抗体的ChIP-seq分析，揭示了TAF4B在精原干细胞中的核心调控作用。结果表明TAF4B直接调控生殖细胞相关基因(如Oct4和Nanog)，其缺失导致干细胞自我更新能力丧失。
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**备注**：以上文献为示例性质，实际引用时需核对具体发表年份及期刊。如需扩展，可进一步检索PubMed或Web of Science数据库，关键词“TAF4B antibody”或“TAF4B function”。
The TAF4B (TATA-box binding protein-associated factor 4B) antibody is a tool used to study the role of TAF4B, a subunit of the TFIID complex, which is critical for RNA polymerase II-mediated transcription. TAF4B, a paralog of TAF4. is distinguished by its tissue-specific expression, particularly in the ovaries, testes, and immune cells. It plays a vital role in reproductive biology, spermatogenesis, oocyte development, and lymphoid tissue function. Studies link TAF4B to transcriptional regulation of genes involved in cell cycle progression, apoptosis, and stress responses. Its dysfunction is associated with infertility, premature ovarian failure, and certain cancers, such as lymphoma.
TAF4B antibodies are designed to detect and quantify endogenous TAF4B protein levels, enabling researchers to explore its expression patterns, interactions, and regulatory mechanisms. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, immunofluorescence, and chromatin immunoprecipitation (ChIP). Commercially available TAF4B antibodies are typically generated in rabbits or mice using immunogenic peptides specific to conserved regions of the human TAF4B protein. Validation includes testing for specificity via knockout controls or siRNA knockdown. Research utilizing TAF4B antibodies has advanced understanding of its role in tissue-specific transcriptional programs and disease pathways, highlighting its potential as a biomarker or therapeutic target in reproductive and immune disorders.
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