| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ATP-dependent RNA helicase DDX42, DEAD box protein 42, RNA helicase-like protein, RHELP, RNA helicase-related protein, RNAHP, SF3b DEAD box protein, Splicing factor 3B-associated 125 kDa protein, SF3b125, DDX42 |
| Entrez GeneID | 11325 |
| WB Predicted band size | 103.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This DDX42 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 104-138 amino acids from human DDX42. |
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以下是关于DDX42 (N-Term)抗体的3篇参考文献及其摘要内容：
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1. **文献名称**：*DDX42 regulates apoptosis and proliferation through a DHX9-dependent pathway in human cells*
**作者**：Li, Y., et al.
**摘要**：该研究利用DDX42 N端特异性抗体，通过Western blot和免疫沉淀技术，揭示了DDX42通过与DHX9相互作用调控细胞凋亡和增殖的机制。实验表明，DDX42在癌细胞中高表达，且其N端结构域对结合RNA和调控下游信号通路至关重要。
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2. **文献名称**：*DEAD-box helicase DDX42 mediates resistance to viral infection by enhancing IFN-β signaling*
**作者**：Zhang, Q., et al.
**摘要**：研究通过免疫荧光和ChIP实验(使用DDX42 N-Term抗体)发现，DDX42通过结合病毒RNA并激活干扰素-β(IFN-β)通路，增强宿主抗病毒免疫反应。DDX42的N端结构域被证实是其与RIG-I信号通路相互作用的关键区域。
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3. **文献名称**：*The role of DDX42 in maintaining genomic stability during DNA replication stress*
**作者**：Wang, L., et al.
**摘要**：本文使用DDX42 N端特异性抗体进行免疫印迹和细胞定位分析，发现DDX42在DNA复制压力下被招募到染色质损伤位点，通过解旋酶活性修复停滞的复制叉。研究强调了DDX42 N端结构域在维持基因组稳定性中的功能。
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注：以上文献为示例性内容，实际文献需通过PubMed或学术数据库(如Google Scholar)以关键词“DDX42 antibody N-terminal”检索获取。若需具体文献，可提供更详细的研究背景或应用场景进一步筛选。
The DDX42 (N-Term) antibody is a research tool designed to target the N-terminal region of the DEAD-box helicase 42 (DDX42) protein, a member of the DEAD-box RNA helicase family. DDX42 is implicated in various RNA-related processes, including transcription, splicing, ribosome biogenesis, and viral RNA sensing. Its conserved DEAD-box domain enables ATP-dependent RNA unwinding, critical for remodeling RNA-protein complexes. The N-terminal region, where the antibody binds, is involved in protein-protein interactions and regulatory functions, making it a key focus for studying DDX42's mechanistic roles.
This antibody is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect DDX42 expression, localization, and interactions. Studies suggest DDX42 localizes to the nucleus, particularly nucleoli, and may regulate cell cycle progression, stress responses, and antiviral defense pathways. Aberrant DDX42 expression has been linked to cancers, neurodegenerative diseases, and viral infections, positioning it as a potential biomarker or therapeutic target. The DDX42 (N-Term) antibody’s specificity for the N-terminal epitope helps distinguish DDX42 from other DEAD-box proteins, ensuring accurate detection in complex biological samples. Validation typically includes knockout cell lines or siRNA knockdown to confirm signal specificity. Its application advances research in RNA biology, virology, and disease mechanisms.
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