| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Polyhomeotic-like protein 3, Early development regulatory protein 3, Homolog of polyhomeotic 3, hPH3, PHC3, EDR3, PH3 |
| Entrez GeneID | 80012 |
| WB Predicted band size | 106.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This PHC3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 819-850 amino acids from human PHC3. |
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以下是关于PHC3抗体的3篇参考文献示例(请注意，以下内容为模拟生成的示例，实际文献需通过学术数据库检索验证)：
1. **文献名称**：*The role of PHC3 in Polycomb repressive complex 1-mediated gene silencing*
**作者**：Wang Y, et al.
**摘要**：研究通过PHC3抗体的染色质免疫沉淀(ChIP)实验，揭示了PHC3在PRC1复合体中的定位及其在调控干细胞分化中靶基因沉默的作用。
2. **文献名称**：*PHC3 antibody-based profiling identifies dysregulation of epigenetic modifiers in cancer*
**作者**：Kimura S, et al.
**摘要**：利用PHC3抗体进行蛋白质组学分析，发现多种癌症中PHC3表达异常与PRC1复合体功能失调相关，提示其作为表观遗传治疗靶点的潜力。
3. **文献名称**：*Characterization of PHC3 knockout mice using PHC3-specific antibodies*
**作者**：Garcia-Martinez V, et al.
**摘要**：通过PHC3抗体的免疫组化和Western blot验证PHC3基因敲除小鼠模型，证明PHC3缺失导致胚胎发育缺陷及Hox基因表达异常。
4. **文献名称**：*PHC3 interacts with EZH2: Co-immunoprecipitation studies using PHC3 antibody*
**作者**：Chen L, et al.
**摘要**：研究使用PHC3抗体进行免疫共沉淀(Co-IP)，发现PHC3与EZH2的相互作用，揭示了PRC1与PRC2复合体协同调控基因表达的机制。
建议通过PubMed或Google Scholar以“PHC3 antibody”或“PHC3 Polycomb”为关键词检索最新文献获取具体信息。
The PHC3 (Polyhomeotic Homolog 3) antibody is a research tool used to detect and study the PHC3 protein, a component of the Polycomb Repressive Complex 1 (PRC1) in mammals. PRC1 is part of the Polycomb group (PcG) protein family, which plays a critical role in epigenetic regulation by maintaining gene silencing through chromatin modification. PHC3. along with PHC1 and PHC2. contributes to PRC1's function in stabilizing higher-order chromatin structures, facilitating histone H2A ubiquitination, and ensuring transcriptional repression of target genes, particularly those involved in development, differentiation, and cell identity.
PHC3 antibodies are widely utilized in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate PRC1 complex dynamics in cellular processes. Researchers employ these antibodies to explore PHC3's role in stem cell maintenance, cancer progression (where PcG dysregulation is common), and developmental disorders. Studies have linked PHC3 to tumor suppression or oncogenic activity depending on context, highlighting its therapeutic relevance. The antibody's specificity and reliability are critical for distinguishing PHC3 from other PHC homologs and validating its interaction partners or post-translational modifications. As epigenetic mechanisms gain attention in disease research, PHC3 antibodies remain essential for deciphering PRC1-mediated gene regulation and its implications in health and disease.
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