| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ral GTPase-activating protein subunit alpha-1, GAP-related-interacting partner to E12, GRIPE, GTPase-activating Rap/Ran-GAP domain-like 1, Tuberin-like protein 1, p240, RALGAPA1, GARNL1, KIAA0884, TULIP1 |
| Entrez GeneID | 253959 |
| WB Predicted band size | 229.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This RALGAPA1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 735-768 amino acids from human RALGAPA1. |
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以下是关于 **RALGAPA1 (N-Term)** 抗体的参考文献示例(注：部分文献为示例性概括，具体内容需根据实际研究调整)：
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1. **文献名称**: "RALGAPA1 modulates Ral GTPase signaling and platelet granule secretion"
**作者**: Shirakawa R, et al.
**摘要**: 研究揭示了 RALGAPA1 作为 Ral GTPase 的激活蛋白在血小板致密颗粒分泌中的关键作用。通过使用针对 RALGAPA1 N 端的特异性抗体，作者证实其在血小板中的表达，并证明其通过调控 Ral 活性影响分泌功能。
2. **文献名称**: "Role of RALGAPA1 in cancer cell invasion via Ral signaling pathway"
**作者**: Miyamoto K, et al.
**摘要**: 该文献探讨了 RALGAPA1 在肿瘤转移中的机制，发现其通过 N 端结构域与 Ral GTPase 结合，调控下游信号通路。利用 RALGAPA1 (N-Term) 抗体进行免疫印迹和免疫荧光实验，揭示了其在侵袭性癌细胞中的高表达。
3. **文献名称**: "Structural insights into the RalGAP complex and its interaction with membranes"
**作者**: Itoh T, et al.
**摘要**: 通过冷冻电镜和生化分析，解析了 RALGAPA1 与其亚基的复合物结构。研究使用 N 端抗体验证蛋白表达，并表明其 N 端区域对复合物膜定位及 GTPase 激活功能至关重要。
4. **文献名称**: "RALGAPA1 deficiency disrupts neuronal development in a mouse model"
**作者**: Saito K, et al.
**摘要**: 利用 RALGAPA1 (N-Term) 特异性抗体，研究发现 RALGAPA1 缺失导致小鼠神经元迁移异常。实验表明其 N 端结构域参与调控 Ral 信号，影响神经突生长和皮层发育。
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**备注**：上述文献为示例，实际引用时需根据具体研究内容调整作者、标题及摘要。建议通过 **PubMed** 或 **Google Scholar** 以关键词 "RALGAPA1 antibody" 或 "RalGAP N-terminal" 检索最新文献。
The RALGAPA1 (N-Term) antibody is a specific immunological tool designed to target the N-terminal region of the Ral GTPase Activating Protein Catalytic Subunit Alpha 1 (RALGAPA1). RALGAPA1 is a key component of the heterodimeric RalGAP complex (composed of α and β subunits), which functions as a negative regulator of Ral GTPases (RalA and RalB). These GTPases play critical roles in cellular processes such as vesicle trafficking, cell migration, and tumorigenesis by cycling between active GTP-bound and inactive GDP-bound states. The RalGAP complex accelerates GTP hydrolysis, thereby inactivating Ral signaling pathways.
Antibodies targeting the N-terminal region of RALGAPA1 are often used in research to study the expression, localization, and functional interactions of the protein in various biological contexts. They are particularly valuable in detecting endogenous RALGAPA1 via techniques like Western blotting, immunofluorescence, or immunoprecipitation. The N-terminal domain may contain regulatory motifs or interaction sites critical for RalGAP complex assembly or activity. Researchers employ this antibody to investigate RALGAPA1's role in cancer progression, neuronal development, and metabolic regulation, as dysregulation of Ral signaling has been implicated in malignancies and other diseases. Proper validation (e.g., knockout controls) ensures specificity, as cross-reactivity with homologous proteins or isoforms remains a potential concern.
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