| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Fanconi anemia group M protein, Protein FACM, ATP-dependent RNA helicase FANCM, Fanconi anemia-associated polypeptide of 250 kDa, FAAP250, Protein Hef ortholog, FANCM, KIAA1596 |
| Entrez GeneID | 57697 |
| WB Predicted band size | 232.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This FANCM antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1752-1783 amino acids from human FANCM. |
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以下是3篇关于FANCM抗体的代表性文献示例(注：文献为假设性示例，建议通过PubMed等数据库检索最新真实文献)：
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1. **文献名称**：*FANCM mediates homologous recombination repair in response to replication stress*
**作者**：Deans, A.J., West, S.C.
**摘要**：本研究利用特异性FANCM抗体进行免疫沉淀和Western blot分析，证明FANCM蛋白通过稳定停滞的复制叉并促进同源重组修复，在复制应激应答中起关键作用。
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2. **文献名称**：*FANCM suppresses meiotic crossover formation by binding to Holliday junctions*
**作者**：Huang, J.W., et al.
**摘要**：通过FANCM抗体的免疫荧光染色和ChIP实验，揭示FANCM在减数分裂中通过结合Holliday junction结构抑制异常染色体交换，维持基因组稳定性。
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3. **文献名称**：*FANCM mutations and associated antibodies in Fanconi anemia diagnosis*
**作者**：Kee, Y., D'Andrea, A.D.
**摘要**：开发了一种高特异性FANCM抗体用于临床检测，证实其在Fanconi贫血患者中识别FANCM基因突变导致的蛋白表达缺失，为疾病诊断提供新工具。
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**建议**：可通过关键词 "FANCM antibody application" 或 "FANCM immunodetection" 在学术数据库中检索最新实验文献，重点关注功能机制或临床诊断相关研究。
The FANCM antibody is a crucial tool in studying the FANCM protein, a central component of the Fanconi anemia (FA) DNA repair pathway. FANCM, encoded by the *FANCM* gene, belongs to the FA complementation group M and plays a pivotal role in maintaining genomic stability by resolving DNA interstrand crosslinks (ICLs) and replication stress. It functions as a DNA translocase, facilitating the recruitment of FA core complex proteins to damage sites, enabling ICL repair via homologous recombination. FANCM also stabilizes stalled replication forks and coordinates replication restart. Mutations in *FANCM* are associated with FA-like disorders and cancer predisposition, though they account for a minority of FA cases.
FANCM antibodies are widely used in research to detect protein expression, localization, and interactions through techniques like Western blotting, immunofluorescence, and immunoprecipitation. These antibodies help elucidate FANCM’s role in DNA repair mechanisms, replication fork dynamics, and its interplay with other FA proteins (e.g., FANCA, FANCD2). Specific FANCM antibodies are validated for specificity using knockout cell lines or siRNA-mediated knockdown. Commercially available monoclonal and polyclonal variants target distinct epitopes, enabling diverse experimental applications.
Research leveraging FANCM antibodies has advanced understanding of FA pathway regulation, cancer biology (particularly in BRCA-related malignancies), and cellular responses to chemotherapeutics like cisplatin. Their use also aids in exploring FANCM’s non-canonical roles in suppressing aberrant homologous recombination and mitigating R-loop accumulation. Reliable FANCM antibodies are essential for dissecting molecular mechanisms underlying genome instability disorders and developing targeted therapies.
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