Rabbit Polyclonal H3f3b Antibody

The H3F3B antibody targets histone H3.3. a variant of the histone H3 family encoded by the H3F3B gene. Histones are core components of nucleosomes, playing critical roles in chromatin structure and epigenetic regulation. Unlike canonical histones (e.g., H3.1 or H3.2), H3.3 is incorporated into chromatin in a replication-independent manner, primarily at transcriptionally active regions, regulatory elements, and sites of DNA repair. This variant is encoded by two distinct genes, H3F3A and H3F3B, which produce identical proteins but differ in regulatory regions and expression patterns. Antibodies against H3F3B are essential tools for studying H3.3-specific functions, including gene expression, cellular differentiation, and genomic stability. They are widely used in techniques like ChIP-seq, immunofluorescence, and Western blot to map H3.3 localization or assess its expression levels. Notably, mutations in H3F3B (e.g., K36M or G34W/R) are linked to cancers such as giant cell tumor of bone and certain gliomas, making these antibodies valuable for diagnostic and research applications. Specificity is crucial, as cross-reactivity with other H3 variants can lead to misinterpretation. Recent studies also explore H3.3's role in aging, reprogramming, and chromatin dynamics, further expanding the utility of H3F3B-targeting reagents in both basic and translational research.