| WB | 1/1000-1/2000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Protein Wnt-8a, wnt8a, wnt-8, wnt8 |
| Entrez GeneID | 30122 |
| WB Predicted band size | 40.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This Zebrafish wnt8a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 135-170 amino acids from the Central region of Zebrafish wnt8a. |
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以下是关于斑马鱼 Wnt8a 抗体的示例参考文献(注：以下内容为虚构示例，仅供参考格式)：
1. **"Generation and characterization of a zebrafish Wnt8a polyclonal antibody for embryonic patterning studies"**
- 作者：A. Smith et al.
- 摘要：该研究报道了一种针对斑马鱼 Wnt8a 蛋白的多克隆抗体的开发，通过重组蛋白免疫兔制备，并验证了其在整胚免疫荧光和 Western blot 中的特异性。抗体成功用于检测早期胚胎中 Wnt8a 在中后脑边界和体节中的表达模式。
2. **"Wnt8a signaling in zebrafish somitogenesis: Insights from antibody-mediated functional inhibition"**
- 作者：J. Lee & C. Kim
- 摘要：研究者利用抗斑马鱼 Wnt8a 的特异性抗体进行功能阻断实验，证明 Wnt8a 在体节形成中调控中胚层分化的作用，抗体通过显微注射干扰内源蛋白活性，导致体节发育缺陷。
3. **"Dynamic expression of Wnt8a during zebrafish neural development revealed by immunohistochemistry"**
- 作者：M. Tanaka et al.
- 摘要：通过抗 Wnt8a 抗体的免疫组化分析，揭示了该蛋白在斑马鱼胚胎神经管闭合和脑区划分中的时空表达变化，为 Wnt 信号在神经发育中的作用提供了新证据。
4. **"Antibody-based screening for Wnt pathway components in zebrafish mutants"**
- 作者：R. Patel & T. Wilson
- 摘要：研究采用 Wnt8a 抗体筛选斑马鱼突变体，发现 Wnt8a 表达水平与特定表型(如尾芽畸形)的相关性，证实了该抗体在表型-分子机制关联研究中的实用性。
(注：实际文献需通过学术数据库检索，如 PubMed、Web of Science 等，关键词 "zebrafish Wnt8a antibody"。)
The zebrafish Wnt8a antibody is a crucial tool for studying the Wnt/β-catenin signaling pathway in developmental biology. Wnt8a, a member of the Wnt family of secreted glycoproteins, plays essential roles in zebrafish embryogenesis, including posterior body patterning, neural plate formation, and somitogenesis. It is particularly implicated in regulating mesoderm specification and establishing the dorsoventral axis during early development.
This antibody is typically generated against specific epitopes of the zebrafish Wnt8a protein, often using synthetic peptides or recombinant proteins as immunogens. It is commonly produced in hosts like rabbits or mice, followed by affinity purification to ensure specificity. Validated applications include immunohistochemistry (IHC), whole-mount in situ hybridization (WISH), and Western blotting, with rigorous testing in zebrafish embryos to confirm minimal cross-reactivity with other Wnt family members.
Research utilizing this antibody has advanced understanding of Wnt8a's dual roles in embryogenesis and tissue regeneration, as well as its dysregulation in disease models. For instance, studies have linked Wnt8a overexpression to developmental defects, while its inhibition can disrupt somitogenesis. The antibody also aids in exploring conserved Wnt signaling mechanisms across vertebrates, bridging zebrafish models to human developmental disorders and cancer. Proper controls, such as Wnt8a-mutant embryos, are recommended to confirm antibody specificity in experimental setups.
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