Mouse Monoclonal DENR Antibody

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     All lanes : Anti-DENR Antibody at 1:1000-1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: human skeletal muscle lysate Lane 3: human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling DENR with P33379 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG   secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin  at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).    

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     Overlay histogram showing Hela cells stained with P33379 (green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33379,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was mouse IgG2b (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

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     P33379 staining DENR in human skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

DENR (Density-regulated protein) is a conserved eukaryotic protein involved in translation regulation and ribosome recycling. It interacts with MCT-1 (Multiple copies in T-cell lymphoma-1) to form a complex that promotes the translation of specific mRNAs, including those with regulatory upstream open reading frames (uORFs). This function links DENR to cellular processes like proliferation, differentiation, and stress responses. Dysregulation of DENR has been implicated in cancers, neurodevelopmental disorders, and viral infection mechanisms due to its role in protein synthesis control.

DENR antibodies are essential tools for studying its expression, localization, and interactions in various biological contexts. Researchers use these antibodies in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate DENR's involvement in tumorigenesis, neuronal development, or viral replication cycles. Some studies highlight DENR overexpression in certain cancers, suggesting its potential as a diagnostic marker or therapeutic target. Additionally, DENR antibodies aid in exploring its partnership with MCT-1 and its impact on mRNA translation fidelity, particularly for oncoproteins or stress-responsive genes. Ongoing research focuses on unraveling DENR's mechanistic roles and validating its clinical relevance in disease models.