| WB | 1/2000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Heat shock cognate 71 kDa protein, Heat shock 70 kDa protein 8, hspa8, hsc70 |
| WB Predicted band size | 71.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This DANRE hspa8 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 528-562 amino acids from the C-terminal region of DANRE hspa8. |
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以下是关于斑马鱼(Danio rerio)HSPA8抗体的假设性参考文献示例，供参考：
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1. **文献名称**: *HSPA8 expression dynamics during zebrafish embryogenesis under stress conditions*
**作者**: Smith J, et al.
**摘要**: 该研究利用特异性HSPA8抗体，通过免疫组化分析斑马鱼胚胎在不同应激(如高温、氧化应激)下的HSPA8蛋白表达变化，发现其在神经管和脊索中显著上调，提示其参与胚胎应激保护机制。
2. **文献名称**: *Role of HSPA8 in zebrafish liver regeneration: Antibody-based functional analysis*
**作者**: Lee S, Kim M.
**摘要**: 通过HSPA8抗体介导的蛋白质抑制实验，揭示了HSPA8在斑马鱼肝脏再生过程中对细胞增殖和凋亡的调控作用，证实其通过PI3K/AKT通路促进组织修复。
3. **文献名称**: *Development and validation of a zebrafish-specific HSPA8 monoclonal antibody*
**作者**: Chen X, et al.
**摘要**: 本研究开发并验证了一种针对斑马鱼HSPA8的单克隆抗体，通过Western blot和免疫荧光证实其高特异性，为后续研究HSPA8在发育及疾病模型中的应用提供工具。
4. **文献名称**: *HSPA8 interacts with tau protein in zebrafish models of neurodegeneration*
**作者**: Garcia R, et al.
**摘要**: 使用HSPA8抗体进行共免疫沉淀实验，发现斑马鱼神经退行性模型中HSPA8与tau蛋白存在相互作用，提示其可能在错误折叠蛋白清除中发挥作用。
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注：以上文献为示例，实际引用需根据真实发表的论文调整。建议通过PubMed或Web of Science以关键词“Danio rerio HSPA8 antibody”进一步检索。
The DANRE (Danio rerio) Hspa8 antibody is a research tool designed to target the heat shock protein family A member 8 (Hspa8) in zebrafish. Hspa8. also known as Hsc70 (heat shock cognate 71 kDa protein), is a constitutively expressed molecular chaperone critical for maintaining cellular homeostasis. It facilitates protein folding, degradation, intracellular trafficking, and stress response by binding to exposed hydrophobic regions of client proteins. In zebrafish, Hspa8 plays essential roles in embryonic development, neurogenesis, and response to environmental stressors, making it a key focus in developmental biology and disease modeling studies.
The DANRE Hspa8 antibody is commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect endogenous Hspa8 expression patterns. Its specificity is often validated through knockout controls or peptide-blocking assays. Researchers employ this antibody to investigate mechanisms of protein quality control, stress adaptation, and Hspa8-associated pathologies, such as neurodegeneration or cancer, in zebrafish models. Cross-reactivity with Hspa8 orthologs in other species may occur due to high sequence conservation, but validation for zebrafish-specific applications is essential. This antibody has contributed to studies exploring cellular stress responses, developmental defects, and toxicology in aquatic organisms, underscoring its utility in both basic and translational research.
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