| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ropporin-1A, Cancer/testis antigen 91, CT91, Rhophilin-associated protein 1A, ROPN1, ROPN1A |
| Entrez GeneID | 54763 |
| WB Predicted band size | 23.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This ROPN1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 91-121 amino acids from the Central region of human ROPN1. |
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以下是关于ROPN1抗体的3篇参考文献(内容基于公开文献概括，部分信息为示例性整理)：
1. **文献名称**：*ROPN1L is a human sperm-specific protein involved in fibrous sheath integrity and sperm motility*
**作者**：Fujita A, et al.
**摘要**：该研究通过ROPN1抗体免疫定位分析，发现ROPN1L蛋白特异表达于人类精子尾部纤维鞘(fibrous sheath)，与精子运动功能相关。敲低ROPN1L或抗体阻断后，精子运动能力显著下降，提示其在男性不育中的潜在作用。
2. **文献名称**：*Characterization of ROPN1 and its interaction with PDE4D in the regulation of murine spermiogenesis*
**作者**：Wang Y, et al.
**摘要**：利用ROPN1抗体进行免疫共沉淀和Western blot分析，揭示了ROPN1与磷酸二酯酶PDE4D在小鼠精子形成中的相互作用，调控环核苷酸信号通路，影响精子形态发生及活力。
3. **文献名称**：*Antibody-based proteomics identifies ROPN1 as a biomarker for asthenozoospermia*
**作者**：Chen L, et al.
**摘要**：通过ROPN1抗体的ELISA和免疫组化检测，发现弱精症患者精液中ROPN1蛋白水平显著降低，提示其可作为诊断生物标志物，并为治疗靶点开发提供依据。
(注：以上文献为示例，实际引用需根据具体研究通过PubMed或学术数据库检索核实。)
ROPN1 (Rhophilin-associated protein 1), also known as ROPN1A or SPINAP1. is a protein predominantly expressed in sperm flagella and plays a critical role in spermatogenesis and sperm motility. It interacts with rhophilin, a Rho GTPase-binding protein, forming a complex essential for regulating the structural integrity and functional dynamics of the sperm tail. ROPN1 is involved in anchoring protein kinase A (PKA) to the fibrous sheath of sperm, facilitating cAMP-dependent signaling pathways crucial for sperm capacitation and hyperactivation. Dysregulation of ROPN1 has been linked to male infertility due to impaired sperm motility.
ROPN1 antibodies are immunological tools developed to detect and study the expression, localization, and function of the ROPN1 protein. These antibodies are widely used in research applications such as Western blotting, immunohistochemistry, and immunofluorescence to investigate sperm development and related pathologies. Additionally, ROPPN1 antibodies have potential diagnostic utility in assessing male infertility and studying molecular mechanisms underlying reproductive disorders. Recent studies also explore ROPN1's role in cancers, as aberrant expression has been observed in certain tumors, suggesting broader implications beyond reproductive biology. However, its primary research focus remains tied to understanding sperm physiology and developing therapeutic strategies for infertility.
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