Mouse Monoclonal RAB3B Antibody

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     All lanes : Anti-RAB3B Antibody at 1:2000 dilution Lane 1: mouse brain lysate Lane 2: rat brain lysate Lane 3: SW480 whole cell lysate Lane 4: human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Overlay histogram showing HepG2 cells stained with P33313 (green line).   The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.   The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33313,   1:25 dilution) for 60 min at 37ºC.   The secondary antibody used was Goat-Anti-Mouse IgG,  DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC.   Isotype control antibody (blue line) was mouse IgG (1μg/1x10^6 cells) used under the same conditions.   Acquisition of >10,  000 events was performed.    

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     P33313 staining RAB3B in human pancreas sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

The RAB3B antibody is a research tool designed to detect and study RAB3B, a member of the RAB family of small GTPases. RAB3B plays a critical role in regulating vesicle trafficking, particularly in secretory pathways, and is highly expressed in neural, endocrine, and exocrine tissues. It is involved in neurotransmitter release, hormone secretion, and membrane fusion events, functioning as a molecular switch that cycles between active (GTP-bound) and inactive (GDP-bound) states. Dysregulation of RAB3B has been implicated in neurological disorders, cancer progression, and metabolic diseases.

RAB3B antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression, localization, and interactions. These antibodies help investigate RAB3B's role in cellular processes, such as synaptic vesicle recycling or exocytosis in secretory cells. Researchers also utilize them to explore RAB3B's post-translational modifications (e.g., prenylation) and its relationship with effector proteins like RIM or Munc-13. In cancer studies, RAB3B antibodies aid in evaluating its potential as a biomarker, as overexpression has been observed in certain tumors. The specificity and validation of these antibodies are crucial, as cross-reactivity with other RAB isoforms (e.g., RAB3A, RAB3C) could lead to misinterpretation. Current research continues to clarify RAB3B's pathophysiological mechanisms and therapeutic potential.