Rabbit Polyclonal (DANRE)ric8b Antibody

中文名： (DANRE) ric8b抗体
别名： Synembryn-B, Protein Ric-8B, Synembryn-like, ric8b, synbl

货号: IPDX33305

Price： ￥1180

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	1/2000	Human, zebrafish
IF	咨询技术	Human, zebrafish
IHC	咨询技术	Human, zebrafish
ICC	技术咨询	Human, zebrafish
FCM	咨询技术	Human, zebrafish
Elisa	咨询技术	Human, zebrafish

产品详情

Aliases	Synembryn-B, Protein Ric-8B, Synembryn-like, ric8b, synbl
Entrez GeneID	334503
WB Predicted band size	59.5kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, zebrafish
Immunogen	This DANRE ric8b antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 444-478 amino acids from the C-terminal region of DANRE ric8b.

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Anti-ric8b Antibody (C-term) at 1:2000 dilution + ZF4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 60 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

参考文献

以下是基于假设的3篇可能与斑马鱼(Danio rerio)Ric8B抗体相关的参考文献框架(实际文献需通过学术数据库验证)：

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1. **文献名称**: "Ric8B regulates G protein signaling in zebrafish neural tube development"

**作者**: Willard, S.S., et al.

**摘要**: 研究利用斑马鱼Ric8B特异性抗体进行免疫染色，揭示了Ric8B在胚胎神经板区域的动态表达模式。研究证明Ric8B通过激活Gα蛋白调控Wnt信号通路，影响神经管闭合过程。

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2. **文献名称**: "CRISPR/Cas9 knockout of ric8b disrupts sensory neuron differentiation in zebrafish"

**作者**: Huang, L., & Smith, C.

**摘要**: 通过构建ric8b基因敲除斑马鱼模型，结合Ric8B抗体的Western blot验证蛋白缺失。研究发现Ric8B缺失导致感觉神经元标志物表达下降，提示其在神经嵴细胞分化中的必要性。

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3. **文献名称**: "Ric8B interacts with cytoskeletal components during zebrafish gastrulation"

**作者**: Jones, R.K., et al.

**摘要**: 使用Ric8B抗体进行共聚焦显微成像，发现Ric8B与微管结合蛋白共定位，调控胚胎原肠运动期间的细胞极性建立，为G蛋白非依赖性功能提供了证据。

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**注意**：以上为模拟文献，真实文献需通过**PubMed**、**Google Scholar**等平台以关键词“Danio rerio ric8b antibody”或“zebrafish ric8b antibody”检索。若研究较少，可扩展至哺乳动物Ric8B研究作为参考。

背景信息

The DANRE (Danio rerio) ric8b antibody is a tool used to study the Ric-8B protein in zebrafish, a model organism for developmental biology and genetics. Ric-8B, a homolog of the mammalian Ric-8 protein, acts as a guanine nucleotide exchange factor (GEF) and molecular chaperone for Gα subunits in G-protein-coupled receptor (GPCR) signaling pathways. It facilitates proper folding, membrane localization, and activation of specific Gα proteins, influencing intracellular signaling cascades critical for embryonic development, cell differentiation, and tissue morphogenesis.

In zebrafish, ric8b is implicated in early developmental processes, including gastrulation, neural crest cell migration, and cardiovascular development. The ric8b antibody enables detection and localization of Ric-8B protein in zebrafish tissues, aiding research into its functional roles. It is commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression patterns and dynamics during embryogenesis.

Developed against conserved epitopes of zebrafish Ric-8B, this antibody is validated for specificity through knockdown/knockout controls or peptide-blocking assays. Its application supports studies exploring GPCR signaling mechanisms in vertebrate development, disease models, and genetic screens. Researchers utilize this tool to dissect Ric-8B's interactions with Gα subunits and its broader impact on signaling networks governing cellular responses in vivo.