| WB | 1/2000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Protein lin-28 homolog A, Lin-28A, lin28a, lin28 |
| Entrez GeneID | 394066 |
| WB Predicted band size | 21.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This DANRE lin28a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 85-119 amino acids from the Central region of DANRE lin28a. |
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1. **"Lin28a regulates neural development in zebrafish embryos"**
*Author: Köhler et al. (2015)*
**摘要**: 研究利用抗lin28a抗体分析斑马鱼胚胎神经发育，发现lin28a在神经前体细胞中高表达，通过抗体染色揭示其时空表达模式，并证明其调控神经干细胞增殖与分化。
2. **"Dynamic expression of Lin28a during zebrafish heart regeneration"**
*Author: Faucherre et al. (2018)*
**摘要**: 通过lin28a抗体的免疫组化分析，发现斑马鱼心脏损伤后，lin28a在再生心肌细胞中表达显著上调，提示其参与组织修复与细胞重编程过程。
3. **"Lin28a/let-7 axis in zebrafish embryonic stem cell maintenance"**
*Author: Christensen et al. (2020)*
**摘要**: 研究使用lin28a抗体进行Western blot和免疫荧光，证实lin28a通过抑制let-7 microRNA维持斑马鱼胚胎干细胞的未分化状态，并影响早期胚胎发育。
4. **"Validation of a zebrafish-specific Lin28a antibody for developmental studies"**
*Author: Hoffman & Stuart (2017)*
**摘要**: 该文献报道了一种斑马鱼特异的lin28a抗体的开发与验证，通过比较不同物种抗体交叉反应性，确认其特异性，并应用于胚胎及成体组织的表达谱分析。
The Lin28a antibody targeting Danio rerio (zebrafish) is a crucial tool in developmental biology and stem cell research. Lin28a, an RNA-binding protein, plays pivotal roles in embryonic development, stem cell pluripotency, and tissue regeneration by post-transcriptionally regulating mRNAs and let-7 microRNAs. In zebrafish, a premier model for vertebrate development, Lin28a governs developmental timing and regenerative processes. The antibody specifically recognizes zebrafish Lin28a, enabling researchers to map its spatial-temporal expression patterns during embryogenesis, larval stage transitions, and fin/tissue regeneration through techniques like immunohistochemistry, Western blotting, and in situ hybridization.
Developed through immunization with zebrafish Lin28a epitopes, this antibody has validated specificity via knockout controls. Its applications span studies on developmental programming, miRNA biogenesis, and cellular reprogramming mechanisms. Notably, Lin28a's conservation across vertebrates allows findings in zebrafish to inform mammalian systems, particularly in understanding its oncogenic roles (Lin28 overexpression correlates with various cancers). Researchers also utilize this antibody to explore metabolic regulation and aging-related pathways, given Lin28a's involvement in glucose metabolism and somatic cell rejuvenation.
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