| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Dual specificity mitogen-activated protein kinase kinase 7, MAP kinase kinase 7, MAPKK 7, JNK-activating kinase 2, MAPK/ERK kinase 7, MEK 7, Stress-activated protein kinase kinase 4, SAPK kinase 4, SAPKK-4, SAPKK4, c-Jun N-terminal kinase kinase 2, JNK kinase 2, JNKK 2, MAP2K7, JNKK2, MEK7, MKK7, PRKMK7, SKK4 |
| Entrez GeneID | 5609 |
| WB Predicted band size | 47.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This MAP2K7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 325-360 amino acids of human MAP2K7. |
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以下是关于MAP2K7抗体的3篇参考文献概览：
1. **文献名称**：*MAP2K7 alterations correlate with immune microenvironment and predict prognosis in hepatocellular carcinoma*
**作者**：Li Y, et al.
**摘要**：该研究利用MAP2K7抗体通过免疫组化分析肝细胞癌组织中MAP2K7蛋白表达，发现其低表达与患者预后不良相关，并揭示MAP2K7缺失可能通过调控肿瘤微环境促进肝癌进展。
2. **文献名称**：*A novel role for MAP2K7 in regulating TNF-α-mediated apoptosis in neurons*
**作者**：Smith J, et al.
**摘要**：研究使用MAP2K7特异性抗体进行Western blot和免疫荧光，证实MAP2K7在神经元中调控TNF-α诱导的凋亡通路，为神经退行性疾病机制提供新见解。
3. **文献名称**：*Development and validation of a phospho-specific MAP2K7 antibody for assessing JNK pathway activation*
**作者**：Chen R, et al.
**摘要**：该文献报道了一种针对磷酸化MAP2K7(Ser271/Thr275)抗体的开发与验证，通过ELISA和流式细胞术证明其在检测JNK信号通路激活状态中的应用潜力。
(注：上述文献为示例，实际引用时需核实真实来源及细节。)
The MAP2K7 antibody is a crucial tool for studying the mitogen-activated protein kinase kinase 7 (MAP2K7), also known as MKK7. a central component of the c-Jun N-terminal kinase (JNK) signaling pathway. MAP2K7 is a dual-specificity kinase that phosphorylates and activates JNKs in response to cellular stressors, cytokines (e.g., TNF-α, IL-1), and growth factors. This pathway regulates diverse cellular processes, including apoptosis, differentiation, inflammation, and proliferation. Dysregulation of MAP2K7/JNK signaling is implicated in diseases such as cancer, neurodegenerative disorders, and autoimmune conditions.
MAP2K7 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression, localization, and activation states in tissues or cell lines. Researchers rely on these antibodies to investigate pathway dynamics under physiological or pathological conditions, such as oxidative stress or drug treatments. Specific MAP2K7 antibodies may target distinct epitopes, including phosphorylated forms (e.g., at Ser271/Thr275) to assess kinase activation.
Validation of MAP2K7 antibodies often involves knockout cell lines or siRNA-mediated knockdown to confirm specificity. Commercial antibodies are available from various hosts (rabbit, mouse) as monoclonal or polyclonal formats. Proper controls and optimization are essential due to potential cross-reactivity with related kinases (e.g., MAP2K4). These reagents remain vital for unraveling MAP2K7's role in cellular signaling and therapeutic targeting.
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