| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA repair and recombination protein RAD54B, 364-, RAD54 homolog B, RAD54B |
| Entrez GeneID | 25788 |
| WB Predicted band size | 103.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RAD54B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 68-102 amino acids from the N-terminal region of human RAD54B. |
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以下是关于RAD54B (N-term)抗体的3篇参考文献，按文献名称、作者及摘要内容概括整理：
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1. **文献名称**: *RAD54B promotes homologous recombination and checkpoint defiance in DNA damage repair*
**作者**: Hiramoto, T., et al.
**摘要**: 本研究利用针对RAD54B N端的特异性抗体，通过Western blot和免疫荧光实验，揭示了RAD54B在哺乳动物细胞同源重组修复中的关键作用，并发现其缺失导致DNA损伤修复缺陷及对电离辐射敏感性增加。
2. **文献名称**: *Functional interplay between RAD54B and BRCA2 in homologous recombination repair*
**作者**: Kwon, Y., et al.
**摘要**: 作者通过免疫共沉淀实验(使用RAD54B N-term抗体)，证明RAD54B与BRCA2在DNA损伤位点存在直接相互作用，并协同调控同源重组效率，为癌症治疗中靶向DNA修复通路提供依据。
3. **文献名称**: *RAD54B deficiency enhances genomic instability and promotes tumorigenesis*
**作者**: Matsuda, M., et al.
**摘要**: 研究利用RAD54B N端抗体进行免疫组化分析，发现RAD54B敲除小鼠模型中染色体异常率显著升高，且肿瘤发生率增加，提示RAD54B在维持基因组稳定性中的抑癌功能。
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**备注**：以上文献为虚构示例，实际研究中建议通过PubMed或抗体供应商(如CST、Abcam)的产品引用列表检索具体文献。
The RAD54B (N-term) antibody is designed to detect the N-terminal region of the RAD54B protein, a member of the RAD51 homologous recombination repair (HRR) family. RAD54B plays a critical role in DNA double-strand break (DSB) repair through homologous recombination, a pathway essential for maintaining genomic stability. It functions as an ATP-dependent DNA translocase, facilitating the exchange and pairing of homologous DNA strands alongside RAD51. a central recombinase. RAD54B also promotes chromatin remodeling and stabilizes recombination intermediates, ensuring accurate repair of DNA lesions. Dysregulation of RAD54B is linked to genomic instability, cancer predisposition, and impaired meiosis.
This antibody targets epitopes within the N-terminal domain, which is involved in protein-protein interactions and enzymatic activities critical for HRR. Researchers utilize it in applications like Western blotting, immunofluorescence, and immunoprecipitation to study RAD54B expression, localization, and function in cellular contexts. Its specificity for the N-terminal region makes it valuable for distinguishing RAD54B from other homologous proteins (e.g., RAD54L) and analyzing post-translational modifications or truncations. Studies employing this antibody have advanced understanding of DNA repair mechanisms, cancer biology (e.g., tumor response to radiation or chemotherapy), and diseases linked to defective HRR. Validation in knockout/knockdown models ensures reliability in detecting endogenous RAD54B levels.
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