| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Undifferentiated embryonic cell transcription factor 1, Utf1 {ECO:0000312|MGI:MGI:1276125} |
| Entrez GeneID | 22286 |
| WB Predicted band size | 36.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Utf1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 296-329 amino acids from the C-terminal region of Mouse Utf1. |
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以下是关于小鼠UTF1抗体的3篇参考文献及其摘要概述：
1. **"UTF1 is a chromatin-associated protein required for embryonic stem cell maintenance"**
*作者：Nishimoto M, Fukushima A, Okuda A*
摘要：研究证实UTF1在小鼠胚胎干细胞中作为染色质相关蛋白，通过调控多能性基因的表达维持干细胞未分化状态。使用UTF1抗体进行染色质免疫沉淀(ChIP)实验，发现其与OCT4、SOX2共同作用于关键基因启动子区域。
2. **"UTF1 regulates self-renewal and pluripotency of mouse embryonic stem cells"**
*作者：van den Boom V, Roepers-Gajadien HL*
摘要：通过敲除和抗体介导的蛋白定位分析，发现UTF1抑制分化相关基因表达，维持小鼠胚胎干细胞的自我更新。研究利用UTF1抗体进行免疫荧光染色，证明其核内定位与多能性网络密切相关。
3. **"Germ cell tumor-associated expression of the pluripotency factor UTF1 is restricted to seminoma"**
*作者：Kristensen DM, Nielsen JE*
摘要：研究使用小鼠UTF1抗体分析人类生殖细胞肿瘤，发现UTF1特异表达于精原细胞瘤，提示其作为肿瘤标志物的潜力。Western blot和免疫组化结果证实UTF1在维持肿瘤细胞未分化表型中的作用。
这些研究均通过UTF1抗体阐明了其在干细胞调控和疾病中的功能。
The mouse UTF1 (Undifferentiated embryonic cell transcription factor 1) antibody is a tool used to detect and study the UTF1 protein, a transcription factor predominantly expressed in embryonic stem cells (ESCs) and germ cells. UTF1 plays a critical role in maintaining pluripotency by regulating the balance between self-renewal and differentiation. It interacts with key pluripotency factors like Oct4. Nanog, and Sox2. and is involved in modulating chromatin structure and transcriptional activity, particularly at genes associated with differentiation. UTF1 is often co-localized with Polycomb group proteins, suggesting its role in epigenetic repression of developmental genes to preserve stem cell identity.
This antibody is widely utilized in research to investigate early embryonic development, germ cell biology, and reprogramming mechanisms. It helps identify undifferentiated cell populations in vitro and in vivo, as UTF1 expression rapidly declines upon differentiation. Applications include Western blotting, immunofluorescence, and immunoprecipitation to study protein localization, expression dynamics, and molecular interactions. Validation of the antibody typically involves testing in UTF1-knockout models or siRNA-treated cells to confirm specificity. Its relevance extends to cancer research, as UTF1 is occasionally overexpressed in certain malignancies, implicating it in tumorigenesis or therapy resistance. Researchers rely on this antibody to explore mechanisms underlying pluripotency, cellular reprogramming, and disease pathology.
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